Background: The study of weed genomics is important for the effective management of weeds to enhance crop yield. A rapid, inexpensive and high quality DNA extraction is needed for genomic and other molecular studies. Here, we describe the protocols for DNA extraction from two different parts of the Echinochloa colona plant using modi ed cetyltrimethylammonium bromide (CTAB) and a commercial kit. Results: In the study, it was observed that the DNA extracted from plant leaf tissues and dry seeds with a modi ed CTAB protocol was of good quality, with no contaminations of polysaccharides and essential oils. Quality of DNA extracted from dry seeds was comparable with that of plant leaves under both protocols. The extracted DNA from both plant parts was successfully ampli ed by PCR using the EPSPS microsatellite marker. Compared to the protocol of DNA extracted from leaf tissue, dry seeds will save time and other valuable resources. Moreover, the same protocols can be implemented for the extraction of highquality DNA for molecular studies in other plant species where a large amount of polysaccharides, secondary metabolites and essential oils are present. Conclusions: Modi ed methods of DNA extraction from dry seeds are e cient and time-saving which can be used in genotypic and other molecular approaches. High-quality DNA can be isolated from plant leaf tissues using modi ed CTAB and commercial kits, however, DNA extracted from dry seeds will save time and other valuable resources.

Smernice v obliki priročnika vključujejo znanja s širšega področja zagotavljanja varnosti, kot so zasebno varovanje, korporativna varnost in pravo javnega naročanja. Publikacija naročnike usmerja k lažjemu in pravilnejšemu izboru ponudnika zasebnovarnostnih storitev, temelji pa na analizi urejenosti področja zasebnega varovanja v kar 32 evropskih državah, na analizi značilnosti delovanja zasebnega varovanja v Sloveniji in na analizi javnega naročanja v Sloveniji. Med drugim priročnik še posebej opozarja na pomen kakovosti zasebnovarnostnih storitev, meril za izbiro ponudnika in primernega razmerja med kakovostjo ter ceno ponujenih storitev na trgu. Vključena so možna merila za naročanje oziroma izbor storitev zasebnega varovanja za različne sektorje (trgovino, javno upravo, industrijo, finančni sektor in fizične osebe-varovanje hiše), ki so pripravljeni specifično za slovenske razmere in so presek teoretičnega razmišljanja ter predlogov in izkušenj strokovnjakov iz gospodarske dejavnosti, na katero se nanašajo.

No abstract

Potato production worldwide is threatened by late blight, caused by the oomycete Phytophthora infestans (Mont.) de Bary. Highly resistant potato cultivars were developed in breeding programs, using resistance gene pyramiding methods. In Sárpo Mira potatoes, five resistance genes (R3a, R3b, R4, Rpi-Smira1, and Rpi-Smira2/R8) are reported, with the latter gene assumed to be the major contributor. To study the level of late blight resistance conferred by the Rpi-Smira2/R8 gene, potato genotypes with only the Rpi-Smira2/R8 gene were selected from progeny population in which susceptible cultivars were crossed with Sárpo Mira. Ten R8 potato genotypes were obtained using stepwise marker-assisted selection, and agroinfiltration of the avirulence effector gene Avr4. Nine of these R8 genotypes were infected with both Slovenian P. infestans isolates and aggressive foreign isolates. All the progeny R8 genotypes are resistant to the Slovenian P. infestans isolate 02_07, and several show milder late blight symptoms than the corresponding susceptible parent after inoculation with other isolates. When inoculated with foreign P. infestans isolates, the genotype C571 shows intermediate resistance, similar to that of Sárpo Mira. These results suggest that Rpi-Smira2/R8 contributes to late blight resistance, although this resistance is not guaranteed solely by the presence of the R8 in the genome.