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Eight stable hybridoma clones derived from fusion of spleen cells of Tobacco Mosaic Virus (TMV) immunized mice with murine myeloma cells were grown in mass culture. They were obtained by 2 subsequent limiting dilution cloning cycles and subcultivation. Five of these clones secreted monoclonal antibodies which reacted with TMV nucleocapsid but not with capsid monomers and the monoclonal antibodies secreted by 3 other clones reacted with the TMV capsid monomer but not with the nucleocapsid. The specificity was determined by Enzyme Linked Immunosorbent Assay (ELISA) adjusted for the detection of antibodies in hybridoma culture supernatants.

Tobacco leaf discs wcrc treated with different concentrations of actinomycin C, DL-parafluorphenylalanine, 8-azaguaninc, chloramphenicol and puromycin-dihydrochloride before, simultaneously and after inoculation with tobacco mosaic virus. The treatment with low concentrations of antimetabolite simultaneously with tobacco mosaic virus inoculation stimulated the tobacco mosiac virus biosynthesis up to ninefold, whereas higher concentrations caused a ten-to iooo-fold inhibition. The stimulatory effect of pretreatment suggests the presence of a factor or factors with antiviral propcrties present in plant tissue becoming activated upon virus infcction. The lack of an effect of treatment with antimetabolites ½ to 3o hr after inoculation indicates that activation of such factor(s) must occur within the first 3o rain. after infection of tobacco leaf cells with tobacco mosaic virus.

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