Humanitarian engineers need an inexpensive, fast, visually compelling way to assess bacterial water quality in remote locations. One way to do this is with 3M Petrifilm E. coli/Coliform (EC) Count Plates to detect E. coli in water samples. These require incubation at close to body temperature. To meet this need, we provide a free, open-source design of a battery-powered incubator capable of maintaining 35°C ± 1°C for up to 65 hours in ambient temperature of 25°C. Our incubator, called the Armadillo, can be replicated by an ordinarily skilled person in five hours for under USD $200.00 in materials cost. This paper summarises the reference documentation for construction, sample handling, inoculation, and incubation using Petrifilms and the Armadillo. Colony-forming unit (CFU) counts generated by the Armadillo are compared side-by-side with a laboratory-grade incubator. Incubation performance at ambient temperatures of 25°C and 4°C shows that a single battery charge reliably powers a full incubation period of 48 hours under normal ambient temperatures.
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