The purpose of this research was to gain an understanding of child consultation in policies for healthy environments in the international evidence base, and examine national and local-level policy and processes regarding child consultation. A systematic mapping review was conducted and grey literature was sought from Aotearoa New Zealand urban and neighbourhood planning, local board, and transport authority websites. A local exemplar project (in Tāmaki Makaurau Auckland City) of child engagement in policy planning was presented. Twenty-four articles met the inclusion criteria for the literature search. The literature was synthesised into three broad themes: co-design and planning processes, green space, and physical activity and body size. Despite the existence of high-level national policies, there was little regional consistency. The 'Healthy Puketāpapa' project presented provided a replicable model for high-quality, local consultation processes. Child consultation is a method through which decision-makers can respect children as citizens, and the process has been found to be beneficial to all stakeholders involved. This research provides an international perspective of literature, and a methodology that can be replicated in other countries and regions for comparability.

Mitosis and homeostasis depend on proper cellular morphological transformation and plasma membrane structure during interphase. PTEN is a tumor suppressor protein involved in cellular homeostasis and genomic stability. We have previously shown that loss of PTEN leads to impairment of mitotic spindle architecture and cytokinesis. Here we report a potential relationship between PTEN and actin in controlling cellular morphology. PTEN depletion results in disorganization of the actin cytoskeleton. This is manifested by decreased parallel alignment of actin filaments, increased F-actin at the membrane, and cells appeared rounder. We also found that Moesin (Moe), an actin-binding ERM protein involved in membrane morphology, exhibits greater level in the phosphorylated Moe (pMoe) form and dislocation of pMoe from the cell cortex. Using an inhibitor of actin polymerization in PTEN-deficient cells, our data show a reduced quantity of pMoe and the recovery of its membrane enrichment. Moreover, inhibition of actin polymerization also reduces F-actin at the membrane and the frequency of cells with round shape. These data suggest that PTEN may regulate cortical actin polymerization to maintain cellular morphology and membrane dynamics.

Role of the Funder/Sponsor: The funding source had no role in the preparation, review, or approval of the manuscript and decision to submit the manuscript for publication. 1.Bennett TD, DeWitt PE, Greene TH, et al. Functional outcome after intracranial pressure monitoring for children with severe traumatic brain injury.