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Purpose of research. To evaluate the effect of LAC (mononuclear blood leukocytes activated by IL-2 ex vivo) suspension infusion on clinical, biochemical, hematological and immunological characteristics of a healthy Beagle dog to assess the safety of the product’s application. Materials and methods. The research object is a 6-year Beagle dog male. The LAC suspension in Hank’s solution has been infused intravenously with a low rate of administration; the dose used was 25 m cells in 5 ml. An autologous culture of LAC has been generated from canine blood, evaluated by morphology, phenotype, and target activity in Laboratory of Cell Immunity by FBSI «N.N. Blokhin National Medical Research Center of Oncology». Results. The analysis of hematological, immunological and radiological characteristics of the object has identified no signs of any pathology development. That turns to conclusion that the given LAC medication dose’s local, hematological and systemic toxicity is low or absent. Conclusions. Data received within the research may be of interest to human medicine as well as to veterinary medicine as it could be considered a part of pre-clinical trials of the LAC suspension, which effectiveness has already been confirmed in human clinical oncology, and also as a safety assessment of even more complex constructs based on activated immunocompetent cells that are still under development. The study can serve as a basis for the development of activated leukocytes-based cellular anticancer immunotherapy (LAC, immune checkpoint molecules and SAR), primarily its toxicity studying models, and also be a part of pre-clinical studies of LAC toxicity.

The review presents the results of three studies of the use of tiletamine-zolazepam for sedation during oral administration in dogs and cats. Study 1. Objective: to study the sedative effect of the combination of tiletamine-zolazepam-acepromazine (TZA) or ketamine-flunitrazepam (CF) in dogs with oral administration, as well as to evaluate the effectiveness of the capsule form of TZA in stray dogs. In a pilot study, 6 dogs were administered a liquid form of TZA (20 mg/kg tiletamine-zolazepam and 2 mg/kg acepromazine) or a liquid form of CF (50 mg/kg ketamine and 2 mg/kg flunitrazepam) orally. Sedation was evaluated in points. On average, the time interval from the introduction of MAL to the adoption of the animal side position was: protocol 1 (infusion of the drug into the mouth) — 47.5 (35...80) minutes (67 % of dogs); protocol 2 (addition of the capsule form to the canned food) — 30 (15...65) minutes (83 %), protocol 3 (addition of the liquid form of the drug in gravy) — 75 (45...110) minutes (100 %). None of the dogs in the CF group in protocols 2 and 3 took a side position. Based on these results, 20 stray dogs received encapsulated MAL: TZ (20 mg/kg) and acepromazine (2 mg/kg). Overall, 63 % of the stray dogs in the experiment were caught after eating a double dose of MAL. Oral administration of encapsulated MAL added to the canned food allows you to catch stray dogs, but it may require the use of additional drugs. The time from the introduction of TZA to the development of sedation in animals and the taste of the drug affect the percentage of capture. Study 2. The purpose of the study is to study the possibility of oral administration of the drug «Zoletil» to cats. 8 healthy cats, females and males, aged from 2 to 4 years, were selected. «Zoletil-50» was used in the form of a sterile lyophilizate prepared in the form of a solution with water for injection in doses from 15 to 25 mg/kg. After administration of the drug, hemodynamic parameters, sedation, and the possibility of endotrachial intubation were evaluated. After oral administration, the effect of sedation developed quite quickly. When administered at a dose of 25 mg/kg, intubation and minor surgical interventions were possible. A dose of 15 mg / kg caused only sedation. It is advisable to use «Zoletil» in the form of a powder with a pleasant taste. Study 3. Objective: to assess the quality of sedation, changes in SAD, heart rate and BH in TB administration of TK to healthy cats. Healthy, sterilized European shorthair cats (n=7; 3 males, 4 females) were included in a randomized, blind, cross-sectional study. Each cat received two doses of tiletamine-diazepam TB: 5 mg / kg (LD) and 7.5 mg/kg (HD) of each drug. TB administration of tiletamine-zolazepam in the studied doses is a simple and effective method of pharmacological fixation of cats, while LD has less effect on SAD and BH than HD.