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Superheated water extraction (SWE) performed in both static and dynamic condition (S-SWE and D-SWE, respectively) was applied for the extraction of essential oil from Thymus vulgaris L. The influence of extraction pressure, temperature, time, and flow rate on the total yield of essential oil and the influence of extraction temperature on the extraction of some chosen components are discussed in the paper. The SWE extracts are related to PLE extracts with n-hexane and essential oil obtained by steam distillation. The superheated water extraction in dynamic condition seems to be a feasible option for the extraction of essential oil components from T. vulgaris L.

The results presented in the paper reveal that MSPD with sand is suitable for the isolation of essential oil components from herbs. Its extraction efficiency is equivalent to pressurised liquid extraction, recognised as one of the most efficient extraction methods. The cost of MSPD procedure for essential oil analysis can be significantly diminished by substituting C(18) with sand.