Cisplatin is a prominent member of the effective broad-spectrum antitumor drugs. However, its clinical usage is restricted due to some adverse side effects, such as testiculototoxicity, hepatotoxicity and nephrotoxicity. The aim of this study is to evaluate the effect of Aqueous Zest Extract of Citrus limonium (AZECL) on the ovary of female Wistar rat treated with Cisplatin. Twenty adult female Wistar rats were divided into four groups (A-D) containing five rats each. Group A rats served as negative control and were treated orally with 2.5 ml/kg body weight of normal saline, group B rats served as positive control group and were treated intraperitoneally with a single dose of 10 mg/kg body weight of Cisplatin, group C rats were treated orally with 50 mg/kg body weight of AZECL and group D rats were treated intraperitoneally with a single dose of 10 mg/kg body weight of Cisplatin and two weeks later treated orally with 50 mg/kg body weight of AZECL. Result showed a significant (p<0.01) decrease in primary follicles, secondary follicles, graafian follicles and a significant (p<0.01) increase in atretic follicles, PAS positive reaction and reduction in the total carbohydrate contents of the stromal cells in the positive control group. Also there was a significant (p<0.05) decrease in the activity level of FSH, LH and a significant (p<0.05) increase in Malondialdehyde when compared to rats in group A and C. The group post-treated with the extract had remarkable normalization of the histo-morphometric, histochemical and biochemical parameters when compared to the positive control group. Aqueous zests extract of C. limonium has a curative effect on cisplatin-induced cytotoxicity on the ovary.

Background: A proper attention is required to prevent the gastric mucosa from injurious effect of aspirin. Objective: We aim to examine the phytochemicals in aqueous extract of Ficus vogelii leaf (F.V.E), and compare their actions on aspirin-induced gastric ulcer with that of Omeprazole. Materials and Methods: Thirty wistar rats (about 8 wks old and weight, 120g each) were divided into 6 groups of five rats each. With the aid of oro-gastric cannula, the groups were administered a daily dose of Omeprazole and F.V.E as follows: group 1 (negative control)-2 ml of distilled water; group 2-Omeprazole 20 mg/kg body weight (bwt) for 10 days, followed by aspirin 500 mg/kg bwt for 3 days; group 3-aspirin 500 mg/kg bwt for 3days, followed by F.V.E 600 mg/kg bwt for 10 days; group 4-aspirin 500 mg/kg bwt for 3 days, followed by Omeprazole 20 mg/kg bwt for 10 days; group 5-F.V.E 600 mg/kg bwt for 10 days, followed by aspirin 500 mg/kg bwt for 3 days; group 6 (positive control)-aspirin 500 mg/kg bwt for 3 days. The rats were sacrificed and the stomach tissues were processed, stained with H & E and PAS, and biochemically analyzed for NP-SH group concentration. Results: F.V.E has high concentrations of saponins and flavonoids. Rats in group 1, 4 and 5 showed normal gastric mucosa. Group 2 and 6 showed gastric ulceration with significant increase in parietal cell count (p<0.01), and reduction in surface cell diameter and count. Group 3 showed evidence of regenerated mucosa. All groups except positive control had normal NP-SH group concentration. Conclusion: The study proved that F.V.E could be used as both therapy and prophylaxis for aspirininduced gastric ulcer in wistar rat while omeprazole adequately served as a therapy for the ulcer.