Energy is vital to the profitable growth of every nation and to stimulate new research. Only natural resources can meet the growing energy demand in recent years, biodiesel has become very interested in the energy as well as environmental advantages that it can be combined with mineral diesel fuel in any quantity. The research focuses on the study of the replacement of diesel with a safflower methyl ester. The engine tests shall be performed using the safflower methyl ester as fuel in the DI diesel engine. The combustion, emission and performance characteristics were studied using alternative fuels and mixtures. SAfflower Methyl Ester 80% (SAME80) and SAME100 have high heat release rates. Nitrogen oxides were higher by about 50%, carbon monoxide decreased by 10%, unburnt hydrocarbon was slightly higher and the thermal efficiency was higher for the SAME than for diesel fuel.