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Background: Mesenchymal stem cells have delivered new approaches to the management of burn healing in severe skin injuries. Aim of the work: To evaluate the effect of bone marrow-derived mesenchymal stem cells [BMSCs] on healing of induced deep 2nd degree skin burns in albino rats. Methodology: 55 male albino rats weighed 200gm were divided into 5 groups, 5 animals in group I [control] and group II [burn model], 15 animals in group III [untreated], group IV and group V [MSCs treated]. Groups III, IV and V were subdivided into 3 subgroups that were sacrificed after 7, 14 and 21 days respectively. Deep 2nd degree burn wounds were induced on a 4 cm2 area on the back of rats in groups II, III, IV and V by brass probes stabilized at 88-90°C and contacted to the shaved dorsal skin of rats for 20 seconds without exerting any external pressure. Mesenchymal stem cells were isolated from the bone marrow of 5 young rats, weighed 100gm then intra-dermally and intraperitoneally injected. Results: By the end of the third week, the wounds of BMSCs-treated groups showed full regeneration of epidermis, reorganization of collagen and decrease in VEGF immunopositive cells. Delayed wound healing was seen in 20% of systemically treated rats. Significant increase in the mean area percent of collagen fibers was detected in topically treated group. Conclusion: Both methods of BMSCs injection were effective in healing of full thickness skin wound but topical method was more effective.

Background: Hyperlipidemia is a disorder in metabolism which means an abnormal increase in levels of lipids (as cholesterol) and lipid-protein in the blood. It is one of the risk factors that mainly cause atherosclerosis, myocardial infarction, coronary heart disease, cerebral stroke, and renal failure. Simvastatin (S) is one of the most prescribed drugs frequently all over the world due to its excellent performance as hypolipidemic and its relatively low price. L-carnitine (LC) is a nutritional supplement supporting with clinical challenges such as dyslipidemia, anorexia & physical performance. This study aimed to evaluate the protective role of LC on the skeletal muscle of hyperlipidemic rats treated with (S). Materials and Methods: Thirty senile male albino rats were used, divided into five equal groups as follows: control group (GI) and the other four groups were fed the high-fat diet for three weeks till the occurrence of hyperlipidemia. GII: was fed the high-fat diet for three weeks (hyperlipidemic) then sacrificed. GIII: hyperlipidemic rats were then treated orally with (S; 1.5 mg/rat/ /day) for 4 weeks. GIV: hyperlipidemic rats were then treated orally with (LC; 20 mg/rat/day) for 4 weeks. GV: hyperlipidemic rats were then treated with (S) and (LC) at the same time. Samples were taken and processed for light and electron microscopic studies. Results: Application of (S) induced multiple changes of muscles as loss of transverse striations, splitting of myofibrils, and presence of central nuclei. Treatment with (LC) causes improvement of these changes. Conclusion: Application of (LC) improved the degenerative changes of muscle obtained by administration of (S).