Hymenoptera venom immunotherapy (VIT) is a treatment which has been shown to be effective in the protection of individuals with systemic allergic reactions caused by Hymenoptera stings. The need for a tool which shows the degree of protection afforded by VIT and the lack of useful biomarkers, has converted the Sting Challenge Test (SCT) into the gold standard for this disorder although its use has both lights and shadows. SCT with Hymenoptera is a test consisting of causing a real sting in a patient diagnosed with an allergy to the venom of the stinging insect and who normally is undergoing treatment with specific immunotherapy. In Spain, SCT is included in the list of services offered by some hospitals and forms part of their habitual clinical practice. This review aims to analyze the strengths and weaknesses of this test and describe the standardized procedure and necessary resources, based on the experience of a group of Spanish experts and a review of the literature.