We used the natural abundance of stable isotopic ratios of hydrogen and oxygen in soil (0.05-3 m depth), plant xylem and precipitation to determine the seasonal changes in sources of soil water uptake by two native encroaching woody species (Pinus ponderosa P. & C. Lawson, Juniperus virginiana L.), and two C(4) grasses (Schizachyrium scoparium (Michx.) Nash, Panicum virgatum L.), in the semiarid Sandhills grasslands of Nebraska. Grass species extracted most of their water from the upper soil profile (0.05-0.5 m). Soil water uptake from below 0.5 m depth increased under drought, but appeared to be minimal in relation to the total water use of these species. The grasses senesced in late August in response to drought conditions. In contrast to grasses, P. ponderosa and J. virginiana trees exhibited significant plasticity in sources of water uptake. In winter, tree species extracted a large fraction of their soil water from below 0.9 m depth. In spring when shallow soil water was available, tree species used water from the upper soil profile (0.05-0.5 m) and relied little on water from below 0.5 m depth. During the growing season (May-August) significant differences between the patterns of tree species water uptake emerged. Pinus ponderosa acquired a large fraction of its water from the 0.05-0.5 and 0.5-0.9 m soil profiles. Compared with P. ponderosa, J. virginiana acquired water from the 0.05-0.5 m profile during the early growing season but the amount extracted from this profile progressively declined between May and August and was mirrored by a progressive increase in the fraction taken up from 0.5-0.9 m depth, showing plasticity in tracking the general increase in soil water content within the 0.5-0.9 m profile, and being less responsive to growing season precipitation events. In September, soil water content declined to its minimum, and both tree species shifted soil water uptake to below 0.9 m. Tree transpiration rates (E) and water potentials (Psi) indicated that deep water sources did not maintain E which sharply declined in September, but played an important role in the recovery of tree Psi. Differences in sources of water uptake among these species and their ecological implications on tree-grass dynamics and soil water in semiarid environments are discussed.

Streambed hydraulic conductivity (K) values were determined at seven stream transects in the Platte River Basin in Nebraska using different instream measurement techniques. Values were compared to determine the most appropriate technique(s) for use in sandy streambeds. Values of K determined from field falling-and constant-head permeameter tests analyzed using the Darcy equation decreased as permeameter diameter increased. Seepage meters coupled with hydraulic gradient measurements failed to yield K values in 40% of the trials. Consequently, Darcy permeameter and seepage meter tests were not preferred approaches. In the upper 0.25 m of the streambed, field falling-and constant-head permeameter tests analyzed with the Hvorslev solution generally had similar K values that were significantly greater than those determined using the Hazen grain-size, Bouwer and Rice slug test for anisotropic and isotropic conditions, and Alyamani and Sen grain-size methods; median differences between these tests and the Hvorslev falling-head 60 cm diameter permeameter were about 8,9, 17, and 35 rnfday, respectively. The Hvorslev falling-head permeameter test is considered the most robust method for measuring K of the upper 0.25 m of the streambed because of the inherent limitations of the empirical grain-size methods and less sediment disturbance for permeameter than slug tests. However, lateral variability in K along transects on the Platte, North Platte, and Wood Rivers was greater than variability in K between valid permeameter, grain-size, or slug tests, indicating that the method used may matter less than making enough measurements to characterize spatial variability adequately. At the Platte River tributary sites, the upper 0.3 m of the streambed typically had greater K than sediment located 0.3 to 2.5 m below the streambed surface, indicating that deposits below the streambed may limit ground waterlsurface water fluxes. The Hvorslev permeameter tests are not a practical measurement approach for these greater depths. Thus, selection of a method for measuring streambed K needs to consider the vertical location of the sediments that are most likely to limit the rate of ground waterlsurface water interaction.

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Naturally occurring long-term mean annual base recharge to ground water in Nebraska was estimated with the help of a water-balance approach and an objective automated technique for base-flow separation involving minimal parameter-optimization requirements. Base recharge is equal to total recharge minus the amount of evapotranspiration coming directly from ground water. The estimation of evapotranspiration in the water-balance equation avoids the need to specify a contributing drainage area for ground water, which in certain cases may be considerably different from the drainage area for surface runoff. Evapotranspiration was calculated by the WREVAP model at the Solar and Meteorological Surface Observation Network (SAMSON) sites. Long-term mean annual base recharge was derived by determining the product of estimated long-term mean annual runoff (the difference between precipitation and evapotranspiration) and the base-flow index (BFI). The BFI was calculated from discharge data obtained from the U.S. Geological Survey's gauging stations in Nebraska. Mapping was achieved by using geographic information systems (GIS) and geostatistics. This approach is best suited for regional-scale applications. It does not require complex hydrogeologic modeling nor detailed knowledge of soil characteristics, vegetation cover, or land-use practices. Long-term mean annual base recharge rates in excess of 110 mm/year resulted in the extreme eastern part of Nebraska. The western portion of the state expressed rates of only 15 to 20 mm annually, while the Sandhills region of north-central Nebraska was estimated to receive twice as much base recharge (40 to 50 mm/year) as areas south of it.