This paper describes a method to preserve functioning islets of Langerhans from mice in Millipore® chamber during eight days of implantation in subcutaneous tissue of normal mice. In vitro it might be difficult to imitate the real conditions which influence the islets of Langerhans (Andersson 1973). Therefore we investigated the possibility of preserving isolated

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During the period from 1982 to 1985, biosynthetic growth hormone (methionyl-hGH) was administered to 55 children with pituitary growth deficiency, 49 with idiopathic, six with other forms of the disorder. Preparations with a relatively high content of Escherichia coli polypeptides (ECP) were given to 32 children (group I), those with a markedly reduced ECP content to 12 (group II), while 11 children received almost ECP-free preparations (group III). The treatment achieved an intensive rise of growth acceleration in all three groups. At the same time, somatomedin C, biological somatomedin activity and alkaline phosphatase rose steeply, reaching a maximum after six months and remaining at high concentrations even thereafter. High antibody titres against ECP and hGH (with high binding capacity) were demonstrated in group I children, significantly lower titres with little binding capacity in those of group II, while most of those in group III had no measurable antibodies. Even in high concentrations the antibodies did not have any inhibiting effect on growth. No positive correlation between antibody titre and growth velocity was demonstrable. An exception was a boy with an allergy in group I, who had a maximal antibody titre and, at the same time, a high serum concentration of IgE. He had marked delay in growth which, however, quickly became normal on administration of extractive hGH.

the mit:odlorrlM.al llleIltrane. With specific methods we meeso.red lipoprotein ani bepatdc lipase activities. Nine newOOm infants were coe-ated en because of gastrotntestdrar an:llBlies . Parenteral nutrition W3.S tullt l4' in three days \olhereafter infants received 3/g/ l<8l day of tat (intro1i pi d) at a constant. rate. 01 the average, weJ$1t gain started at ttle age of 5 days ani eaa 16 g/day . The durat100 of parenteral rutr iti cn was .1-23 weeks. Dring the first week lipoprotein lipase activity Inc-eased trail 1~to 35 }"Ol ffAlml./h 100tlereas hepatd c l ipase activity rem1ned at lIDJ=l ffAlml./h .Jtritioo. Serun free cam1tine decreased trail 25 to 11 )lIXll / l ani acylcam1tine trail 9 to 2 i=l/l l /ng of creatinine. Serun tr1glycerldes, tree fatty acids and blood betao.hydro~tyrate rezained. , ha.lever , practically~Wring parenteral IUtritioo. The results _ t t1a t neither lipoprotein lipase actfvrty rce-cam1tine availability are rate-l imiting for the utd .Hzatd cn of tat in newbern i nfants duri ng parenteral IUtr i t ioo.