This study presents a list of angiosperm species in an Atlantic Forest fragment in the southern portion of Espírito Santo state, Brazil, a region that represents a collection gap within the Atlantic Forest. The studied site is a relatively small fragment of 144 ha located within a conservation unit, the Mata das Flores State Park. The site belongs to a conservation priority area for the Atlantic Forest in Espírito Santo, and is under strong anthropic pressure. Of the 239 species listed here, 21 are new records for the state, eight are endemic, and 20 figure either in the country’s or the state’s Red Lists of endangered species. Rubiaceae and Piperaceae were the families with the highest number of species. We show that small fragments that were never inventoried before can reveal a relatively large number of threatened species and that collection gaps need to be filled in order to refine our understanding about conservation priorities within the Atlantic Forest Biome.

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