Investigations were carried out concerning the considerate and selective separation of intact, product including recombinant E. coli cells from culture broths by adsorption. Adsorbents were synthesized on basis of porous glass ''SIRAN'' (Schott, Mainz) by chemical surface modification in order to adapt surface charge density and hydrophobicity to the surface behavior of the hosts. It is possible to accumulate up to 58 mg dry biomass per gram carrier by using the fixed bed circulation technique and by simultaneous dosage of small amounts of polycationic reagent Polyethyleneimine (PEI). The method is especially useful for the separation of shear stress sensible microorganisms.Preferred release of the recombinant product staphylokinase (SAK) from the cytoplasmatic space of the adsorbed cells was done by permeabilization of the cells and elution of the column with suitable agents.Thus, product enrichment was achieved simultaneously with the clearence of host cell proteins and other cell components or fragments.

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