First and fourth larval instars of a sensitive laboratory strain of the cotton leaf worm; Spodoptera littoralis Boisd. were reared on castor Ricinus communis L. leaves; treated with three concentrations (5.0, 10.0 and 15.0%) of water extracts prepared from three different varieties of mulberry leaves; Morus alba var rosa, M. alba var morittiana and M. alba var kokuso -27. Different biological aspects were determined to evaluate the insecticidal effect of the three mulberry varietyleaves. The M. alba var rosa was significantly more lethal to both larval instars of S.littoralis than the M. alba var morittiana and M. alba var kokuso-27 The fourth larval instar was more tolerant to the lethal effect of all mulberry leaf extracts than the first one. The higher the concentration of mulberry leaf extracts the greatest the lethal effect obtained with all treatments. Protein profile of haemolymph samples was monitored for the sixth larval instar of S.littoralis leaves by the 15.0% concentration of all mulberry varieties from the fourth larval instar. Electrophoretic protein analysis showed disappearance of some protein bands in haemolymph samples from treated larvae by M. alba var rosa.
Water deficit is the main yield-limiting factor in Egypt and overall the world. Thus the main objective from this work was to evaluate eighteen barley genotypes differed in their genetic makeup under six varied environments which are the combination between, two growing seasons (2013/2014 and 2014/2015) and three water irrigation levels (800, 1100 and 1400 m 3 /fad.) as severe stress, moderate stress and adequate, respectively in newly reclaimed sandy soil of South El-Qantara Shark, Ismailia, Egypt, for days to 50% heading, flag leaf area, plant height, 1000-grain weight, biological yield and grain yield. The combined analyses of variance and the mean square of joint regression analysis of variance revealed significant differences among genotypes (G), environments (E) and the G × E interaction for all the traits. Environment + Genotype × Environment (E + G × E), mean squares due to environment (linear) and linear interaction (G × E linear) had highly significant effects for all characters. Severe and moderate water stress levels were significantly reduced all studied traits except 1000-grain weight for all barley genotypes than adequate water supply treatment. Grain yield reduced in the 1 st year by an average of 25.67% and 13.44% and in the 2 nd year by an average of 26.82% and 12.67% under 1 st and 2 nd irrigation levels, respectively, compared with the 3 rd level. Phenotypic stability parameters showed that barely genotypes, Line 9, Rihane 3 and Line 11 were highly adapted to favorable environments for days to 50% heading; Line 6, Line 5 and Giza 2000 for plant height; Giza 126 for flag leaf area; Line 1, Line 11and Rihane 3 for 1000-grain weight; Line 9, Line 7 and Line 2 for biological yield and Line 6, Line 9 and line 10 for grain yield. Genotypic stability parameters indicated that barley genotypes Line 11 and Giza 2000 was highly adapted to favorable environments for days to 50% heading; Line 2 for plant height, 1000-grain weight and biological yield; California mariout, Giza 123, Line 4, Line 6 and Line 7 for grain yield. The additive main effects and multiplicative interaction (AMMI) analysis of variance showed highly significant difference among genotypes, environments, G × E, IPCA1 and IPCA2. AMMI stability value (ASV) and GE biplot revealed that, the most desired and stable genotypes were Line 1, Giza 123, California mariout, Line 8 and Line 6 for grain yield. According to genotype plus genotype × environment (GGE) biplots, the ideal genotype was Line 9 for days to 50% heading; Line 6 for plant height; Line 11 for flag leaf area; Line 1 for 1000-grain weight; Line 9 for biological yield and Line 6 for grain yield.
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