Microbial biodegradation of polycyclic aromatic hydrocarbons (PAHs) like Anthracene, represent an efficient, time, and cost-effective way for bioremediation of the polluted environment. This study was aimed at isolating, identifying and characterizing bacteria with potential to degrade and utilize anthracene as a sole carbon source. A bacteria was isolated from oil spilled contaminated site located in Kano, using an enrichment method on mineral salt media (MSM) following serial dilution (10-1-10-6). Characterization was done by studying the effects of temperature and pH on mineral salt media (MSM) containing anthracene. The isolate was then identified morphologically, biochemically and molecularly based on 16S rRNA partial gene sequence analysis. The morphological and microscopic examination of the isolate from this research shows that the isolate was creamy in color, motile, gram negative, short rod and non-spore forming respectively. The biochemical test of the isolate was found to be positive for these parameters (methyl red, catalase, motility, indole and urease) and negative for (citrate and oxidase). The 16S rRNA sequence and Phylogenetic analysis using neighbor joining tree and 1000 boos trap revealed that, the isolate was closely related (on the same clade) to Proteus vulgaris with accession number MW766369. Characterization was done by studying the effects of temperature and pH. The isolated bacterium was optimal at a temperature of 35ºC and pH 7.5. This isolate is a promising strain that could be used in bioremediation of polycylic aromatic hydrocarbons polluted environment.

Taman Nasional Bukit Barisan Selatan (TNBBS) merupakan habitat bagi jenis-jenis tumbuhan berbunga unik, salah satunya yaitu kantong semar. Kantong semar (Nepenthes sp.) merupakan tumbuhan karnivora pemangsa serangga yang dilindungi oleh pemerintah Indonesia dan termasuk ke dalam Appendiks II dalam CITES. Saat ini, data mengenai keanekaragaman jenis kantong semar dan persebarannya di TNBBS masih sangat minim, serta belum adanya penelitian mengenai kantong semar khususnya di Resort Muara Sahung. Penelitian ini bertujuan untuk mengidentifikasi keanekaragaman jenis dan sebaran kantong semar di Resort Muara Sahung, Taman Nasional Bukit Barisan Selatan, Kabupaten Kaur, Bengkulu. Serta menganalisis karakteristik habitatnya. Identifikasi jenis kantong semar dilakukan dengan melakukan perbandingan dengan literatur. Penentuan lokasi penelitian menggunakan metode purposive sampling dan dilakukan marking untuk mendapatkan data sebaran. Karakteristik habitat diidentifikasi dengan analisis vegetasi serta pengukuran komponen abiotik. Terdapat tiga jenis kantong semar yang teridentifikasi, yaitu Nepenthes gymnamphora, Nepenthes spathulata, dan Nepenthes ovate. Kantong semar tersebut tersebar di ujung utara Resort Muara Sahung pada ketinggian lebih dari 1700 m dpl. Karakteristik habitat kantong semar yang ditemukan di Resort Muara Sahung yaitu berada di ketinggian di atas 1700 m dpl, suhu 17,91 °C, kelembaban 88,83%, tutupan tajuk 41,167%, dan pH sebesar 5.

Taman Nasional Bukit Barisan Selatan (TNBBS) merupakan habitat bagi jenis-jenis tumbuhan berbunga unik, salah satunya yaitu kantong semar. Kantong semar (Nepenthes sp.) merupakan tumbuhan karnivora pemangsa serangga yang dilindungi oleh pemerintah Indonesia dan termasuk ke dalam Appendiks II dalam CITES. Saat ini, data mengenai keanekaragaman jenis kantong semar dan persebarannya di TNBBS masih sangat minim, serta belum adanya penelitian mengenai kantong semar khususnya di Resort Muara Sahung. Penelitian ini bertujuan untuk mengidentifikasi keanekaragaman jenis dan sebaran kantong semar di Resort Muara Sahung, Taman Nasional Bukit Barisan Selatan, Kabupaten Kaur, Bengkulu. Serta menganalisis karakteristik habitatnya. Identifikasi jenis kantong semar dilakukan dengan melakukan perbandingan dengan literatur. Penentuan lokasi penelitian menggunakan metode purposive sampling dan dilakukan marking untuk mendapatkan data sebaran. Karakteristik habitat diidentifikasi dengan analisis vegetasi serta pengukuran komponen abiotik. Terdapat tiga jenis kantong semar yang teridentifikasi, yaitu Nepenthes gymnamphora, Nepenthes spathulata, dan Nepenthes ovate. Kantong semar tersebut tersebar di ujung utara Resort Muara Sahung pada ketinggian lebih dari 1700 m dpl. Karakteristik habitat kantong semar yang ditemukan di Resort Muara Sahung yaitu berada di ketinggian di atas 1700 m dpl, suhu 17,91 °C, kelembaban 88,83%, tutupan tanjuk 41,167%, dan pH sebesar 5.

The widespread and long term use of atrazine results in high residue levels of atrazine in soil, which further causes water contamination, it is considered as an endocrine disruptor and is potentially carcinogenic. Microbial degradation of herbicide represents a time cost effective way of eco-restoration. This research was aimed at isolating and characterizing bacteria capable of degrading and utilizing atrazine as a sole carbon source. An enrichment method was used to isolate the bacteria on mineral salt media (MSM) following serial dilution. The isolate was identified morphologically, biochemically and molecularly as Bacillus safensis strain BUK_BCH_BTE6 based on 16S rRNA gene sequence and molecular phylogenetic analysis. The effect of pH and temperature on the degradation of atrazine was studied in MSM medium supplemented with atrazine as sole carbon source. Growth and degradation of atrazine in this isolate was optimal at pH 7.5 and temperature of 35 °C. Bacillus safensis is highly efficient in atrazine degradation with an optimum range of pH and temperature. Bacillus safensis could be a suitable candidate for bioremediation of atrazine polluted sites.

Chromium (Cr) is toxic, mutagenic, carcinogenic, and harmful to most plants, animals, and bacteria inhabiting aquatic environments because it is motile, highly toxic, soluble in water and it is a good oxidizing agent and it is detrimental to cell membranes. Chromium remediation with the help of microorganisms is believed to be the best and cheaper technology at hand to clean up Cr contamination, some chromium resistant bacteria can reduce chromium (VI) to (III) with the help of reductase enzymes. This research was aimed to isolate and identify bacteria capable of reducing chromium from tannery effluent. The level of chromium from different tannery industries was analyzed using Microwave Plasma Atomic Absorption Spectroscopy (MPAES) and compared to Environmental Protection Agency (EPA) standard which shows that all the samples were above the EPA standard except for one sample, a pour plating method was used to isolate the bacteria on Luria Bertani (LB) amended with K2Cr2O7 after serial dilution, the isolate was identified morphologically, biochemically and molecularly as Acetanaerobacter sp. based on 16s rRNA gene sequencing and molecular phylogenetic analysis, Atomic absorption spectrophotometry was carried out to determine the level of chromium from the bio-reduced sample. A reduction of 18.32% was observed in broth treated with the isolate within 48 hours of incubation at 37°C.