Among 504 out-patients (males and females) suffering from various forms of sexually transmitted diseases at special treatment clinic in University College Hospital, Ibadan, 48 (9.5%) of them were found to have Trichomonas viginalis. Trichomoniasis was found to be more prevalent in women (12.5% of 280), thirteen (5.8%) out of the 224 males had trichomoniasis with a high prevalence in the age bracket of 11 -30 years in both sexes. Large percentage of single women and men investigated (53% and 26% respectively -79% in all based on isolates) had trichomoniasis. It was discovered that 68.4% of patients infected with trichomoniasis had discharge (vaginal and urethral), 42.1% had pain on passing urine while 10.5% had irritation on passing urine, and also 10.5% had increase frequency. Twenty-two (42%) of 48 patients with T. vaginalis were diagnosed by microscopy of direct wet mount preparation, all the 48 (100%) positive cases were diagnosed by culture. The two media (Trichomonas medium 2 (Oxoid) and NBGS supported the growth of T. vaginalis and they failed to show superiority over each other. (Afr. J. Biomed. Res. 10: