The influence of applied nitrogen (N) (120-720 kg/ha · year) on Lolium perenne (perennial ryegrass) vegetative, reproductive, and aerial tiller densities was monitored during the establishment year under grazing. Total (vegetative, reproductive, and aerial) tiller densities ranged from 3500 tillers/m2 (at 120 kg N/ha · year) during April 1994 to 17 800 tillers/m2 (at 600 kg N/ha · year) during August 1994. Increasing levels of N (up to 360 kg N/ha · year) increased total tiller density. Reproductive tillers were observed in November and December 1993 and ranged from 6 (at 600 kg N/ha · year) to 27 (at 480 kg N/ha · year) tillers/m2. Nitrogen levels above 240 kg/ha · year promoted reproductive tiller development, whereas levels above 600 kg/ha · year depressed reproductive tiller development. Since the low reproductive tiller densities resulted from frequent intensive grazing, little practical importance can be attached to these results. Aerial tiller densities ranged from 12 (at 480 kg N/ha · year) in January to 487 (at 720 kg N/ha · year) tillers/m2 in September 1994. Increasing levels of applied N increased aerial tiller density, whereas at low levels (120 and 240 kg N/ha · year) aerial tillering was negligible.

Lolium perenne L. (perennial ryegrass) exhibits poor persistence in subtropical environments. Grazing management may enhance the vigour and hence persistence of this species. Perennial ryegrass was subjected to various grazing treatments, and its vigour, indexed by etiolated growth, was evaluated over 2 years. Pasture and individual tiller vigour were monitored under 5 combinations of grazing frequency and intensity, applied rotationally, and 1 treatment of continuous grazing. The vigour of infrequently grazed plots was greater than that of frequently or continuously grazed plots; however, grazing intensity did not influence vigour. Seasonally, vigour declined during mid (December and .January) to late (February and March) summer of the establishment year and from early summer (October) to autumn (May) during the second year. Poor vigour in frequently grazed plots was associated with low growth reserves rather than a lack of active tiller growth points. Towards the end of the second year, however, a lack of active tiller growth points also limited vigour in frequently grazed plots. Tillers from infrequently grazed plots (regardless of grazing intensity) had greater vigour than tillers from the frequently grazed plots. To enhance the vigour of perennial ryegrass in subtropical environments, the frequency of grazing should be reduced, particularly in summer.

Changes in pasture metabolisable energy (ME), crude protein (CP), neutral detergent fibre (NDF), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), sodium (Na) and chlorine (Cl) were measured at each grazing, in 4 paddocks on 2 farms (farm A and farm B) in western Victoria from May 1995 to March 1997. Selection differentials were calculated from quality parameters using measures of pre- and post-grazing masses. Changes in botanical composition and pasture allowance were also measured. The nutritive characteristics of pasture on both farms followed similar trends. Metabolisable energy and CP were highest in winter and early spring with values of over 11 MJ/kg DM and 250 g/kg DM respectively. The lowest concentrations of ME and CP in pasture on farm A (9.4 MJ/kg DM; 128 g/kg DM) were observed in April and February respectively. On farm B the corresponding lowest values (8.4 MJ/kg DM; 100 g/kg DM) were in March. In contrast, NDF values on farm A were lowest in June (466 g/kg DM) and on farm B (436 g/kg DM) in May, with highest values in February (648 g/kg DM) and March (692 g/kg DM) respectively. Concentrations of P, K, S and Cl in pasture followed a similar pattern to that of ME with highest values in winter and lowest in summer, while changes in Ca concentrations related more closely to changes in NDF. The concentrations of Mg and Na were highest in autumn and spring, respectively, with lowest values in late spring and summer. Selection differentials indicated that the ME of pasture consumed was 4–22% higher than the pasture on offer. For CP the range of selection differentials was always greater than 1, but varied widely (1.08–1.83). The selection differentials for NDF were always negative, with an average value of 0.84. Selection differentials for minerals varied little over the year, with values generally indicating a positive selection differential (P 1.12, 1.15; Ca 1.16, 1.16; Mg 1.15, 1.18; K 1.23, 1.27; Na 1.05, 1.07; Cl 1.16, 1.14; S 1.27, 1.28) for farms A and B. Pasture allowances ranged from 10–60 kg DM/cow.day and there was no relationship between selection differentials for all nutritive characteristics and pasture allowance. The results obtained in this study indicated that although the ME of pasture consumed throughout the year should be adequate to meet production of 30 L/cow.day in early lactation with minimal liveweight loss, it was associated with high levels of CP and potentially low concentrations of NDF in relation to cow requirements. Results therefore indicate a possible need for supplementing pasture diets in winter and spring to balance the diet for CP and NDF. In addition, it is likely that both Ca and Mg intake from pasture may be limiting in early lactation and therefore additional supplements of both minerals may be required during this period.

A 3-year experiment determined the impact of multiple applications of different rates of nitrogen (N) fertiliser, applied over autumn and winter in 1997, 1998, and 1999, on N2 fixation in grazed dryland perennial ryegrass (Lolium perenne)/white clover (Trifolium repens) dairy pastures. Four treatments replicated 3 times in a randomised block design comprised: 0 N (A); 3 applications of 25 kg N/ha (B); 3 applications of 50 kg N/ha (C); and 3 applications of 75 kg N/ha (D). Urea (46% N) was the N source and applications were from autumn to late winter. 15N abundance methods, based on herbage samples, were used to estimate N2 fixation. Percentage N derived from the atmosphere (%Ndfa) through white clover N2 fixation decreased as the rate of N fertiliser application increased during 1997, but remained unaffected by treatment during 1998 and 1999. Slight decreases in %Ndfa, associated with an increase in soil nitrate, were offset by the positive association between clover dry matter (DM) and soil ammonium. The amount of N2 fixed by white clover decreased from 43 kg N/ha.year (A) to 23 kg N/ha.year (C) during 1997. The amount of N2 fixed by white clover during 1998 and 1999, however, was unaffected by increasing rates of N fertiliser application and averaged 17 and 12 kg N/ha.year in 1998 and 1999, respectively. White clover sward composition (percentage of DM) was unaffected by the rate of N fertiliser application. The average white clover composition across treatments declined from 23% DM in 1997 to 12% DM in 1999. It is concluded that the DM yield production of dryland perennial ryegrass/white clover dairy pastures is likely to be restricted by available soil N, as the amount of N2 fixed annually by clover is low (approx. 20 kg N/ha.year). There is, therefore, scope to supplement pasture N nutritional requirements with fertiliser N. Further, application of N fertiliser may restrict the ability of white clover to fix N2; however, under the grazing management adopted, N fertiliser had no effect on the percentage of white clover.

Summary. At 2 sites in south-western Victoria, 132 plots of predominantly perennial ryegrass pasture were randomly allocated, within 4 replicate blocks, to each of 3 lock up dates (L1, L2, L3) by 12, 12 or 9 harvest times. Harvesting commenced 2 weeks after initial treatment lock up with L1 and L2 being harvested 12 times (weekly intervals) and L3, 9 times. Lock up dates were 15 August (L1), 5 September (L2) and 26 September (L3) at site 1 and 17 August (L1), 7 September (L2) and 28 September (L3) at site 2. For each treatment and harvest date, dry matter yield and botanical composition were determined and samples of total pasture and the ryegrass fraction were collected and assessed for dry matter digestibility, crude protein and neutral detergent fibre. Dry matter yield was measured from the start of L1 (site 1, 15 August; site 2, 17 August) until the final harvest date of L3 (site 1, 12 December; site 2, 14 December). At site 1, L3 produced higher dry matter yields than L1 and L2 at comparable lengths of lock up time, whilst there were no differences at site 2. Over the total experimental period (site 1, 15 August–12 December; site 2, 17 August–14 December) there were no differences in total dry matter yield (t/ha) between treatments at either site (site 1—L1 5.79, L2 6.43, L3 5.94; site 2—L1 6.68, L2 5.07, L3 5.73). Treatments had little effect on botanical composition at either site when compared at the same time after lock up, both during the harvesting period or in the subsequent autumn. Pasture metabolisable energy and crude protein all declined with increasing length of lock up whilst neutral detergent fibre content increased, changes which were similar for both the total pasture and the ryegrass fraction. The metabolisable energy of pasture in L1 and L2 was higher than that of L3 at least until week 8 at both sites. Initial crude protein values were higher for L1 and L2 than for L3 at site 1, whilst at site 2, L1 had higher values than either L2 or L3. Although longer lock up periods produced more herbage, if conserving forage is to be an integral component of managing surplus spring pasture, then dairy farmers should aim to produce high quality pasture for forage conservation. This will be achieved through shorter lock up periods and harvesting pasture no later than early ear emergence in the ryegrass fraction of the sward. This management will reduce dry matter yields, but allow more flexibility for maintaining intensive grazing practices through the spring period. The decision about when to lock up pasture will depend on both plant growth rates and animal feed requirements.