GC-MS with negative ion chemical ionization has been used to detect anabolic estrogens (diethylstilbestrol, dienestrol, hexestrol, and 17p-and 17a-estradiol) in bovine muscle tissue. After hydrolysis and extraction, purification by column chromatography, and derivatization, the detection limit for the estrogens is better than 0.1 ppb.
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