Rosacea is a chronic cutaneous inflammatory disease that affects mostly the facial skin, commonly more observed in caucasic population. Although, its pathophysiology is still not cleared it has been theorized to be caused by immunologic alterations and neurovascular dysregulation. Because of the complexity of rosacea, for a diagnosis, it can be categorized into four mainly clinical presentations: papulopustular, erythematotelangiectatic, ocular, and phymatous, but phenotypic presentations of rosacea are more heterogeneous leaving the diagnosis unclear, mistaken or even, and commonly can be confused with another type of disease. Currently, it is suggested, that there is a difference in temperature between the skin with rosacea and the normal skin, but this information is still not confirmed. Our aim is to establish an individual diagnosis criteria based in the difference of thermal patterns of healthy patients and patients with rosacea. This work was a single-blinded controlled study, where thirty healthy patients and twelve patients with rosacea underwent on application of four solutions with different lipid consistence and his temperature changes were monitored by digital infrared thermography. The results, depending on the solution applied, shows a statistically different temperature changes on the two kind of face surface, and so, we conclude that the digital infrared thermography could be used as an additional noninvasive medical diagnosis tool to rosacea detection. 489Dupilumab efficacy in atopic dermatitis in four randomized phase 3 trials (liberty ad solo 1&2, chromos, cafe) Dupilumab (DUP), a fully human IL-4Ra mAb, inhibits signaling of IL-4 and IL-13, key drivers of type 2/Th2-mediated inflammation. DUP is approved in the EU, USA, and other countries for the treatment of adults with inadequately controlled moderate-to-severe atopic dermatitis (AD). Our objective is to present efficacy and safety data from four phase 3 trials; pooled SOLO 1&2 (NCT02277743, NCT02277769 ) -DUP monotherapy vs placebo (PBO) in patients (pts) with moderate-to-severe AD; CHRONOS (NCT02260986) -DUP administered concomitantly with topical corticosteroids (DUP+TCS) vs PBO+TCS in pts with moderate-tosevere AD; and CAFÉ (NCT02755649) -DUP+TCS vs PBO+TCS in pts with severe AD not adequately controlled with, intolerant to, or medically inadvisable for oral cyclosporine A (CSA). Pts were randomized to PBO, DUP 300 mg every 2 wks (q2w), or DUP 300 mg qw for 16 wks in all trials. More DUP-treated pts vs PBO achieved an investigator's global assessment score 0/1 and 2-point improvement at Wk16 in all trials: pooled SOLO (37%/37% vs 9%; q2w/qw vs PBO), CHRONOS (43%/41% vs 12%), and CAFÉ (40%/39% vs 14%) (P<0.001 for all). Similar results were observed for % change in eczema area severity index and peak pruritus numerical rating scale scores respectively: pooled SOLO (-70%/-71% vs -34% and -47%/-49% vs -21%), CHRONOS (-81%/-82% vs -48% and -57%/-57% vs -31%), and CAFÉ (-80%/-78% vs -47% and -54%/-52% vs -25%) (P<0.001 for all). In all tria...

Acne is a skin disease with chronic and inflammatory characteristics that is present on the pilosebaceous unit appearing first at puberty and often in adulthood. Its pathophysiology involves hyperseborrhea, non-normal follicular-based keratinization, and Propionibacterium acnes increase in the pilosebaceous unit resulting in a change in the cutaneous microenvironment leading to inflammatory reactions. 1 Ablative fractional laser (AFL) surgery is widely used in the medical field because it is able to reduce trauma due to the short-time exposure to radiation. 2 Isotretinoin has been considered for several decades the gold standard for nodulocystic acne or inflammatory acne, which has failed other course treatments, due to its anti-inflammatory properties. 3It has been established that combining the use of AFL surgery with oral isotretinoin improvement is observed on acne scars in a short period of time with no acne relapse or adverse effects over the resurfaced skin. 4-8 Abstract Background: Ablative fractional laser surgery is a common technique for treating acne scars. However, an in vivo and noninvasive analysis of the histologic variations between acne skin and the resulting resurfaced skin is needed in order to evaluate the wound healing process of the scars induced by the ablative fractional laser surgery. Materials and Methods: Nine patients with acne scars underwent a single treatment with a CO 2 ablative fractional laser surgery. Collagen presence on the resurfaced skin was noninvasively assessed by means of Raman spectroscopy and principal component analysis.Results: Principal component analysis shows that all the patients presented a collagen regeneration on the resurfaced skin after the laser treatment. Conclusion:Collagen plays a crucial role in the wound healing process. By assessing the collagen presence on the skin, it was possible to quantify the regenerative effects of the ablative fractional laser in a noninvasive way.

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Nanoheaters are promising tools for localized photothermal therapy (PTT) of malignant cells. The anisotropic AuNPs present tunable surface plasmon resonances (SPR) with ideal NIR optical response to be applied as theranostic agents. To this purpose, nanoparticles with branches are suitable because of the electromagnetic field concentrated at their vertices. We standardized a protocol to synthesize multibranched gold nanoparticles (MB-AuNPs) by the seed-growth method and found a size-seed dependence tunability on the hierarchy of branching. Once the optical response is evaluated, we tested the temporal stability as nanoheaters of the MB-AuNPs immersed in skin-equivalent phantoms by dynamic infrared thermography (DIRT). The most suited sample presents a concentration of 5.2×108 MB-AuNPs/mL showing good thermal stability with ΔT = 4.5°C, during 3 cycles of 10 min at 785 nm laser irradiation with power of 0.15 W. According to these results, the MB-AuNPs are suitable nanoheaters to be tested for PTT in more complex models.

Non-invasive medical diagnosis has become popular due to the possibility of detecting illnesses in vivo and in real time this technique, often referred to as "optical biopsy", comprises several optical techniques such as thermography, diffuse reflectance spectroscopy, optical coherence tomography and Raman spectroscopy among others. Particularly Raman spectroscopy is an optical technique based on the inelastic scattering of light that can detect disease markers, this technique has been successfully used to detect several types of diseases, however the high price of a Raman spectrometer makes it difficult for the medical community to adopt its use as a common diagnostic procedure. In this work a Raman spectroscopy system was designed and fabricated from low-cost readily available components. The system was characterized and the Raman spectra obtained was compared to commercial systems. Results show that it is possible to fabricate a custom Raman system with the desired optical configuration for non-invasive optical diagnosis at low costs and portable size.