This study introduces the mainstream theory of crowd evacuation based on multi-agent. Then a simulation model is developed based on Agent and Pathfinder to simulate the evacuation of three subway space types, including platform, station hall and stair. In addition, simulation is also carried out on the situation of the reverse running of the firefighters walking ladder and the different distribution of the crowd density. Finally, the simulation parameters are adjusted respectively to observe and compare the simulation results under different circumstances. Through the simulation of the situation of crowd evacuation under different conditions and parameters, key data such as crowd speed, number of people passing through space and remaining number, number of people passing through exit and evacuation path are recorded. These key data and the above simulation test results are vital for constructive suggestions that can be put forward for subway space design and fire control scheme.