El objetivo del presente trabajo fue evidenciar la presencia del circovirus porcino 2 (PVC2) y la serorreactividad en granjas porcícolas en las regiones de Tolima y Huila, Colombia, mediante un estudio transversal. Se tomaron muestras de sangre y tejidos (linfonodos, pulmón y riñón) de animales de todas las etapas productivas, incluyendo animales sanos y con signos de la enfermedad. Mediante la prueba de ELISA se detectaron animales seropositivos en todas las etapas productivas. Para identificar la presencia del virus se amplificó el ORF2 completo del PCV2 mediante PCR punto final utilizando ADN extraído de sangre, linfonodos, pulmón y riñón. Las muestras fueron genotipificadas, encontrándose animales positivos a PCV2, tanto sanos como enfermos, en todas las etapas de producción. El análisis de las secuencias demostró un porcentaje de identidad entre 93 y 99% con el genotipo PCV2d. Se encontraron animales sin signos clínicos positivos a la prueba de ELISA y al PCR, lo cual sugiere la presencia de infecciones subclínicas o variaciones en la virulencia de las cepas infectantes. Adicional a estas pruebas, se realizó una encuesta epidemiológica, donde no se detectaron asociaciones significativas entre la presencia del virus y el manejo de la producción.

No abstract

Background and Aim: The oviduct environment is of particular importance because it is the site of fertilization and early embryo development. The oviduct, as a component of the reproductive system, responds to ovarian hormone (estradiol [E2] and progesterone [P4]) stimuli depending on the estrous cycle phase. This study aimed to elucidate the effect of estrous cycle phases (follicular and early and late luteal phases) on gene expression patterns in bovine oviduct epithelial cells (BOECs). Materials and Methods: Oviducts were obtained from healthy slaughterhouse animals, corresponding to ipsilateral ovaries with dominant follicles or corpus luteum during early and late luteal phases. BOECs were recovered from the isthmus (IST) and ampulla (AMP), and the expression patterns of genes related to cytokinesis and mitosis mechanisms (rho-associated coiled-coil containing protein kinase and cellular communication network factor 2 [CCN2]), growth factors (insulin-like growth factor-binding protein 3, epidermal growth factor receptor [EGFR], vascular endothelial growth factor A, and EGFR), antioxidant mechanisms (glutathione peroxidase 4 [GPX4]), apoptosis (B-cell lymphoma 2), complement component (C3), energy metabolism (aldose reductase gene family 1-member b1 [AKRIB1] and solute carrier family 2), hormone receptors (estrogen receptor 1 and luteinizing hormone/choriogonadotropin receptor), and specific glycoproteins (oviductal glycoprotein 1) were analyzed. Results: High P4 levels (late luteal phase) affected the expression of important genes related to antioxidant mechanisms (GPX4), energy metabolism (AKRIB1), growth factors (IGBP3 and EGFR), and cell growth regulation (CCN2) in the AMP. Low P4 levels (early luteal phase) affected the expression of AKR1B1, IGBP3, and CCN2. In addition, estrogen likely had an effect on OVPGP expression in the cattle oviduct. Conclusion: Differential gene expression patterns of BOECs in the AMP during the luteal phase (antioxidant mechanisms, energy metabolism, growth factors, and immunological regulators) and in the IST during the follicular phase (glycoproteins) may influence their renewal and population proportions, modulating the oviduct environment as well as gamete and embryo physiology.