The genus Paracoccidioides comprises known fungal pathogens of humans and can be isolated from different infection sites. Metabolic peculiarities in different members of the Paracoccidioides led us to perform proteomic studies in the presence of the two-carbon molecule acetate, which predominates in the nutrient-poor environment of the phagosome. To investigate the expression rates of proteins of different members of Paracoccidioides, including one isolate of P. lutzii (Pb01) and three isolates of P. brasiliensis (Pb03, Pb339, and PbEPM83), using sodium acetate as a carbon source, proteins were quantified using label-free and data-independent liquid chromatography-mass spectrometry. Protein profiles of the isolates were statistically analyzed, revealing proteins that were differentially expressed when the fungus was cultivated in a non-preferential carbon source rather than glucose. A total of 1,160, 1,211, 1,280, and 1,462 proteins were reproducibly identified and relatively quantified in P. lutzii and the P. brasiliensis isolates Pb03, Pb339, and PbEPM83, respectively. Notably, 526, 435, 744, and 747 proteins were differentially expressed among P. lutzii and the P. brasiliensis isolates Pb03, Pb339, and PbEPM83, respectively, with a fold-change equal to or higher than 1.5. This analysis revealed that reorganization of metabolism occurred through the induction of proteins related to gluconeogenesis, glyoxylic/glyoxylate cycle, response to stress, and degradation of amino acids in the four isolates. The following differences were observed among the isolates: higher increases in the expression levels of proteins belonging to the TCA and respiratory chain in PbEPM83 and Pb01; increase in ethanol production in Pb01; utilization of cell wall components for gluconeogenesis in Pb03 and PbEPM83; and increased β-oxidation and methylcitrate cycle proteins in Pb01and PbEPM83. Proteomic profiles indicated that the four isolates reorganized their metabolism in different manners to use acetate as a carbon source.

SUMMARY:Inadequate ossification of the interparietal region induces the appearance of interparietal and wormian bones, also associated with genetic factors. The formation of the interparietal bone depends on the separation of the intermediate segment from the lateral plate by the transverse occipital suture, which means that this bone is formed by the medial and lateral plates. Wormian interparietal bones or epactal bones are located within the interparietal region, being single or multiple, and are located in the upper central region of the interparietal region, the sutural bones, however, are formed from additional ossification centers that can occur in near sutures. The aim of this work was to macroscopically evaluated the presence of changes in the structure of the adult human occipital bone in cadavers found in the human anatomy laboratory of the Goiás Federal University in order to determine the frequency of supernumerary bones associated with occipital and parietal bones and relate it to literature, as well as to provide images and data for ethnic studies in the Brazilian population and data that could help medical practices such as fetal position and radiographs. Our data have indicated that the frequency of the interparietal bone of 1.92% is among those seen in countries such as Japan, Bulgaria, Nigeria, India and Turkey, but lower than values found in pre-Hispanics Chileans; however, for the sutural bones, the frequency of this study was higher than other data obtained from Spanish and other Brazilians.