This work presents the application of the NIR technique associated with exploratory analysis of spectral data by main principal components for the discrimination of Amazon cocoa ground seeds. Cocoa samples from different geographic regions of the state of Pará, Brazil (Medicilândia, Tucumã, and Tomé-Açu), were evaluated. The samples collected from each region were divided into four groups distinguished by the treatment applied to the samples, which were fermented (1-with fat and 2-fat-free) and unfermented (3-with moisture and 4-dried). Each set of samples was analyzed separately to identify the influence of moisture, fermentation, and fat on the geographical differentiation of the three regions. From the results obtained, it can be observed that it was not possible to differentiate the samples of seeds not fermented by geographic origin. However, fermentation was crucial for efficient discrimination, providing more defined clusters for each geographic region. The presence of fat in the seeds was a determinant to obtain the best model of geographic discrimination.
bem como a concentração de extrato enzimático (0,50; 1,00; 1,50; 2,00; 2,50 e 3,00%). Monitorou-se a atividade da LOX pelo acompanhamento do aumento da absorbância a 234 nm durante 240 segundos, expressos em milimolar por segundo por grama (mM/s.g). Ambos os ácidos graxos estudados mostraram picos de atividade de LOX em pH 6,5 e concentração de substrato a 1,5 mM. Aplicando-se os dados experimentais à equação de Michaelis-Menten e da linearização de Lineawer-Burk, os valores obtidos para a constante de MichaelisMenten (K M ) e de velocidade enzimática máxima (V MAX ) variaram entre: 0,164 a 0,169 mM e 0,083 a 0,093 mM/s.g, respectivamente. O ácido linoleico revelou-se o melhor substrato para a enzima e foi utilizado para determinação da atividade da LOX em função da concentração de enzima, que apresentou maior atividade a 3,00%.
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