Pepper is one of the most important vegetables and spices in the world. Principal pungency is contributed by secondary metabolites called capsaicinoids, mainly synthesized in the placenta of pepper fruit. Various factors, including drought, limit pepper production. Flowering is one of the most sensitive stages affected by drought stress. The current study was conducted to determine the effect of drought on different pepper genotypes at the flowering and pod formation stages. Hot pepper (Pusajuala and Ghotki) and Bell pepper (Green Wonder and PPE-311) genotypes were subjected to drought (35% field capacity) at two different stages (flowering (DF) and pod formation (DP) stage). In comparison, control plants were maintained at 65% field capacity. The data regarding flowering survival rates, antioxidant protein activity, and proline content, were collected. Results indicated that parameters like flower survival percentage, number of fruits per plant, and fruit weight had significant differences among the genotypes in both treatments. A high proline level was observed in Green Wonder at the pod formation stage compared to other genotypes. Capsaicin contents of hot pepper genotypes were affected at the pod formation stage. Antioxidants like GPX were highly active (190 units) in Ghotki at pod formation. Bell pepper genotypes had a high APX activity, highly observed (100 units) in PPE-311 at pod formation, and significantly differ from hot pepper genotypes. In the catalase case, all the genotypes had the highest values in DP compared to control and DF, but Pusajuala (91 units) and Green Wonder (83 units) performed best compared to other genotypes. Overall, the results indicate that drought stress decreased reproductive growth parameters and pungency of pepper fruit as most of the plant energy was consumed in defense molecules (antioxidants). Therefore, water availability at the flowering and pod formation stage is critical to ensure good yield and pepper quality.
Severe water stress conditions limit growth and development of floricultural crops which affects flower quality. Hence, development of effective approaches for drought tolerance is crucial to limit recurring water deficit challenges. Foliar application of various plant growth regulators has been evaluated to improve drought tolerance in different floricultural crops; however, reports regarding the role of chitosan (Ci) on seasonal flowers like calendula are still scant. Therefore, we evaluated the role of Ci foliar application on morphological, physiological, biochemical, and anatomical parameters of calendula under water stress conditions. Different doses of Ci (0, 2.5, 5, 7.5, 10 mg L−1) were applied through foliar application to evaluate their impact in enhancing growth and photosynthetic pigments of calendula. The optimized Ci level of 7.5 mg L−1 was further evaluated to study mechanisms of water stress tolerance in calendula. Ci application significantly increased biomass and pigments in calendula. Ci (7.5 mg L−1) resulted in increased photosynthetic rate (72.98%), transpiration rate (62.11%), stomatal conductance (59.54%), sub-stomatal conductance (20.62%), and water use efficiency (84.93%). Furthermore, it improved catalase, guaiacol peroxidase, and superoxide dismutase by 56.70%, 64.94%, and 32.41%, respectively. These results highlighted the significance of Ci in inducing drought tolerance in pot marigold.
The assessment of heterotic F1 combinations is a basic requisite for hybrid seed development. A set of 30 F1 hybrids along with their parental inbred lines were evaluated under both normal and water deficit conditions for various physiological and agronomic traits. Highly significant mean squares due to general combining ability, specific combining ability and reciprocal effect were observed for all traits under both water regimes. Components of variation exhibited greater estimates for GCA variance (б 2 g) than SCA variance (б 2 s) for majority of the traits under both normal and stress conditions depicting the predominant role of additive genetic component.
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