Thirty-one Aman rice germplasm were evaluated to assess their morphological variability and genetic diversity considering ten quantitative and thirty one qualitative traits at the field experimental plot of Bangladesh Institute of Nuclear Agriculture, Mymensingh, following randomized complete block design. The germplasm showed significant differences for all the studied traits. The highest grain yield/plant was recorded in Sentu-16 whereas the lowest was found in Gaindha. The genotypic coefficient of variation (GCV) was lower than those of the corresponding phenotypic coefficient of variation (PCV) for all the traits. High PCV and GCV along with heritability, genetic advance (GA) and GA in percentage of mean were recorded for number of filled grains/panicle and number of unfilled grains/panicle. Grain yield/plant had positively significant correlation with plant height, number of total tillers/hill, number of effective tillers/hill, number of filled grains/panicle and thousand grain weight. Path co-efficient analysis suggested that grain yield/plant had positive direct effect in association with days to fifty percent flowering, number of effective tillers/hill, panicle length, number of filled grains/panicle and thousand grain weight. Principal component analysis revealed that the first three components accounted for 79.57% of cumulative variance. Cluster analyses were revealed that the studied rice germplasm organized into five clusters based on D2 values where maximum inter-cluster distance was observed between cluster II and V. Qualitative characterization by distinctness, uniformity and stability test revealed that a wide range of variation was observed among the genotypes. Therefore, this study would be useful for breeders to choose and identify the revival and preservation of beneficial genes for crop improvement.
Lentil is a leguminous crop which plays important role in both human health and agriculture. Lack of genotypic and phenotypic variability limits the scope of breeding for developing high yielding lentil varieties. In order to know the genotypic and phenotypic variability and diversity in lentil, research was carried out with 30 lentil genotypes following a randomized complete block design using three replications. Highly significant variability was found for all of the traits among the genotypes under study. The genotype ILL 4127 showed superior performance whereas ILL 2894 showed poor performance. The environment had great impact on the genotypes as the PCV (phenotypic coefficient of variation) was greater than the GCV (genotypic coefficient of variation). High PCV coupled with high GCV, heritability, genetic advance and genetic advance in percentage of mean was found for number of pods plant -1 and number of seeds plant -1 . Thirty genotypes were divided into 5 clusters where cluster I and cluster V had the maximum inter-cluster distance, specifying the existence of wider genetic diversity among the genotypes of these clusters. Hence, suitable transgressive segregants might be found from crosses between genotypes of those clusters. Genetic diversity at molecular level was found 0.861 to 1.112 which indicated the existence of genetic variation within the studied genotypes. Microsatellite profiling revealed that SSR19 was the highly informative and detectable polymorphic marker followed by SSR48, SSR156, SSR33, SSR13 and SSR130. The results showed the consistency both in molecular and morphological clustering.
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