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This study determined the potential risk factors that may contribute to seropositivity among dogs and dog handlers from working dog and dog shelter institutions. Data was collected from dogs (n = 266) and dog handlers (n = 161) using a standardised guided questionnaire. Serum obtained from the dogs and dog handlers was tested using the microscopic agglutination test (MAT). A logistic regression analysis was used to predict leptospiral seropositivity of dogs and dog handlers based on potential risk factors. A total of 22.2% of dogs and 21.7% of dog handlers were seropositive. The significant predictors for the dogs’ seropositivity were presence of rats (OR = 4.61 (95% CI: 1.05, 20.33), p = 0.043) and shared common area (OR = 5.12 (95% CI: 1.94, 13.46), p = 0.001) within the organisation. Significant predictor for dog handler seropositivity was contact time with the dogs of more than six hours/day (OR = 3.28 (95% CI: 1.28, 8.40), p = 0.013) after controlling for the effect of other risk factors such as small mammal contact, rat infestation at home, flooding at housing area (within three months) and urban locality. The exposure to various disease sources identified poses risk to dogs and dog handlers. Risk could be reduced with adequate application of protection at work while handling dogs and thus limiting contact with these sources and reducing exposure to infection.

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The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass and production of exopolysaccharide (EPS) of Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) was applied and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p < 0.0001) for both mycelium biomass and EPS production. The yeast extract concentrations and initial pH showed the strongest effect (p < 0.001) meanwhile sucrose concentrations showed significant value at p < 0.005 for mycelium biomass. For EPS production, only sucrose and yeast extract concentrations gave significant value with p < 0.001 and p < 0.005 respectively. The model was validated by applying the optimized conditions and 25.8 ± 0.9 g/L of mycelium biomass, and 5.7 ± 0.4 mg/mL EPS concentration was obtained. The estimated optimum conditions of the variables for the production of mycelium biomass and EPS production by Lentinus squarrosulus are as follows: sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81; sucrose concentration 115.8 g/L, yeast extract of 3.39 g/L and initial pH of 6.44 respectively.

Background: Dengue fever is endemic in Malaysia and continues to be a public health concern. Selangor was the top hit and there is a paucity of information on knowledge, attitudes, and practices (KAP) of Bandar Baru Bangi residents regarding dengue infection. Therefore, the objective of this study is to describe dengue related knowledge, attitudes and practices (KAP) of residents of Bandar Baru Bangi.Methods: A cross-sectional questionnaire survey of 152 residents from Section 3 and Section 8, Bandar Baru Bangi attending the dengue awareness events was conducted. The information on socio-demographic characteristics of the participants and their knowledge, attitude and practice on dengue fever was collected using a structured questionnaire.Results: The results showed that the residents only 52% had sufficient knowledge about the dengue vectors, signs, symptoms, and modes of transmission. However, approximately all the respondents considered dengue as serious but preventable disease to which they are vulnerable to. Television/radio was the predominant sources of information about dengue fever. Knowledge on dengue is associated with prevention practice. But the self-efficacy has a significant impact between the owner and tenant house (p=0.010, p<0.05). Female has higher self-efficacy compare to male.Conclusions: As conclusion, findings suggest that despite the residents of Bandar Baru Bangi have moderate knowledge level about dengue fever nevertheless; they still adopted the preventive measures suggested by the guidelines from Ministry of Health. Therefore, health program planners and practitioners need to identify why the dengue fever still rampant among the population.