Background The recent pandemic by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global emergency. There is large number of asymptomatic cases of SARS-CoV-2 that are not reported. Hence, serological evidence of SARS-CoV2 antibodies is warranted for a better estimation of the actual number of infected patients to limit the disease spread and to get an idea of herd immunity. Methods This is a cross-sectional study conducted from May 2020 to July 2020 at National Institute of Blood Diseases at Pakistan. The study includes healthcare workers (HCWs), community and industrial workers. The anti-SARS-CoV-2 test was performed by electrochemiluminescence immunoassay analyzer. Results A total of 1675 samples have been received from three groups of population. The percentage positivity for industrial employees is high (50.3%) for HCW (13.2%) and community population (34%).Total percentage for positive antibodies result is ~36%. Conclusion Our seroprevalence is 36%, which still far from herd immunity that needs to be at least 60–70% in population. If we consider acquiring 60% seroprevalence in next few months, then herd immunity is not far from reality, provided the antibodies did not decline with time. Although the current study is based on a small sample of participants, the findings suggest a study with larger population to implement stronger and targeted interventions.

Introduction: A high MPV on admission in septic patients may be a useful indicator associated with poor outcome especially when it is associated with decreasing platelet numbers. Objective: To determine the frequency of mortality in neonates with sepsis having high MPV. Material & Method: Study design: Descriptive case series study Settings: NICU, Shifa International Hospital, Islamabad Methodology: All newborns of both genders up to 28th day of life who have culturally proven sepsis or probable sepsis with gestational age of ≥28 weeks, birth weight > 700gms were enrolled. The initial investigations on admission was carried out as per NICU septic screening protocol includes complete blood count CBC, C-Reactive Protein (CRP), electrolytes, renal function tests, blood C/S, urine R/E urine C/S, x-ray chest while the other investigations including CSF analysis, gram staining and culture, tracheal secretions and catheter tips for culture and sensitivity are performed when indicated clinically. Outcome was measured till discharge or death. Results: Mean age of the patients was mean ±SD 6.58 ±5.72 days. Most of the neonates 59 (62.1%) were males whereas 36 (37.9%) were females. Mean CRP, TLC count, platelet count, TLC count at baseline were 21.23 ±42.34, 15190.5 ±4619.32, 254348.42 ±101479.87 respectively. MPV at baseline 10.56 ±0.85 and at 48 hours was 9.96 ±0.81 respectively. Mortality was observed in 22 (23.2%) neonates. Conclusion: The frequency of mortality was found to be 23.2% in neonates with sepsis having high MPV. Keywords: Mortality, Neonates, Sepsis, Mean Platelet Volume