Grasses on the Pakistani coast are moderately to highly salt tolerant and have potential for utilization as a cash crop. This study was designed to determine whether seed germination of three halophytic grasses (Phragmites karka, Dichanthium annulatum and Eragrostis ciliaris) could be improved by exogenous application of ascorbic acid (AsA) under saline conditions. Seeds of P. karka were germinated in varying concentrations of NaCl and AsA under different temperature regimes, and seeds of Dichanthium annulatum and Eragrostis ciliaris were germinated at optimal temperatures only. In P. karka, concentrations of AsA (5 and 10 mM) alleviated the salinity effects better at cooler and moderate thermo‐periods, whereas higher concentrations (20 mM of AsA) failed to improve germination under all temperature regimes. AsA was ineffective at a warmer thermo‐period (25/35°C). The rate of germination also increased at all thermo‐periods with the application of AsA except at 25/35°C under saline conditions. Application of AsA improved the germination of E. ciliaris seeds under saline conditions but was inhibitory for D. annulatum in comparison with the untreated control. The rate of germination followed the similar pattern as that of seed germination. Results indicate that AsA has the ability to partially alleviate the effect of salinity on seed germination of some grass species under optimal temperature regime.
Urochondra setulosa (Trin.) C.E. Hubbard is a coastal halophytic grass thriving on the coastal dunes along the Pakistani seashore. This grass could be useful in coastal sand dune stabilization using seawater irrigation. The purpose of this investigation was to test the hypothesis that Ca 2+ (0.0, 2.5, 5.0, 10.0 and 50.0 mmol/L) alleviates the adverse effects of KCl, MgSO 4 , NaCl and Na 2 SO 4 at 0, 200, 400, 600, 800 and 1000 mmol/L on the germination of Urochondra setulosa. Seed germination was inhibited with increase in salt concentration with few seeds germinated at and above 400 mmol/L concentration. No seed germinated in any of the KCl treatments. Inclusion of CaCl 2 substantially alleviated the inhibitory effects of all salts. Germination was higher under photoperiod in comparison to those seeds germinated under complete darkness. Among the CaCl 2 concentrations used, 10 mmol/L was most effective in alleviating salinity effects and allowing few seeds to germinate at 1000 mmol/L KCl, MgSO 4 , NaCl and Na 2 SO 4 solution.
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