The current study was carried out to investigate the toxic effects of aflatoxin B1 exposure on heart, lung, and testis of male Albino rats. Twelve healthy male Albino rats were used in the current study. They were divided equally into 2 groups (control and treated groups). The treated group was given aflatoxin B1 orally by a gastric tube at concentration 0.5 mg per kg of body weight for 7 day. The control group was given distilled water alone. At the end of experiment (1 week), the rats were killed under ether anesthesia and the internal organs(heart, lung, and testis) were collected immediately for histological examination. The histopathology examination in heart revealed that there is oedema between myocardial. In lung, there is a large number of inflammatory cells and dilated the alveolar sac with exudates. The testis revealed the excessive vacuolated cells and suppression spermatogenesis. In conclusion, oral dose of aflatoxin B1 exposure could induce a deleterious effect on the histological structure of the heart, lung, and testis of male Albino rats. High level of attention must be taken to prevent the possible contamination of our food with aflatoxin B1.