P olymorphism is defined as the simultaneous occurrence of two or more discrete, genetically-based phenotypes in a population, in which the frequency of the rarest type is higher than can be maintained by recurrent mutation (McKinnon and Pierotti 2010). Species that exhibit visible polymorphisms are ideal for examining the microevolutionary forces that maintain genetic variation in nature, because phenotypes are easily scored and because color or pattern polymorphisms often exhibit simple Mendelian inheritance (Hoffman and Blouin 2000). In anurans, polymorphism for red, green, or brown/gray dorsal color is a common motif, with some species exhibiting a combination of the above; for example, variation in the Pacific Treefrog (Hyla regilla) includes the presence/absence of dorsal stripes as well as an array of ground colors (Resnick and Jameson 1963). The anuran family Rhacophoridae comprises 440 currently recognized species and the genus Nasutixalus contains three species distributed in India (West Bengal and Arunachal Pradesh) and China (southeastern Xizang) (Frost 2021).Jerdon's Treefrog (Nasutixalus jerdonii) was first described by Günther (1876) from Darjeeling, West Bengal, and is known to occur in India (West Bengal, Nagaland, Manipur, and Meghalaya), Bhutan, and Myanmar (Putao District, Kachin State) (Frost 2021) (Fig. 1). Sankar et al. (1992) and Sankar and Ray (2006) provided brief accounts for West Bengal and Arunachal Pradesh. Biju et al. ( 2016) rediscovered the species and studied its biology from Mawphlang Secret Grove in Meghalaya and gave a detailed distribution of the species from India (Nagaland, Manipur, and Meghalaya).At 2116 h on 10 May 2021, employing the Acoustic Encounter Survey (AES) system, we collected a single male Jerdon's Treefrog (MZMU 2400) during a herpetofaunal survey at Hmuifang Community Reserve Forest, and another male (MZMU 2533) at 2010 h on 12 September 2021 from the same locality (23°27'17.14"N, 92°45'10.76"E; elev. 1,471 m asl). Both were calling from a tree-hole 7 m above the ground in secondary forest in a park constructed by the Department of Local Administration. The tree-hole was 47 mm in diameter, 110 mm in depth, and one-third filled with water. On 13 May 2021, a forest patrol encountered another frog in Murlen National Park, Champhai District, Mizoram (23°39'43.06"N, 93°17'25.47"E; elev. 1,521 m asl). All were preserved and deposited in the Departmental Museum of Zoology, Mizoram University (MZMU). Photographic vouchers were submitted to the Natural History Museum of Mizoram, Mizoram University, Aizawl, Mizoram, India (NHMM/G/9-4). We amplified the partial 16s rRNA gene using primers L02510 (Palumbi 1996) and H03063 (Rassmann 1997) and compared it to 21 congeneric sequences retrieved from the NCBI database. We aligned the