The equivalent conductivities of proline in water, methanol were studied in the temperature range of 288.15 to 313.15 K at 5 K intervals, in mixtures of methanol and water at percentages of 10 %, 20%, 30%, 40% and, 50% of methanol at 37°C. The experimental data were treated by the Lee-Wheaton conductivity equation of unsymmetrical electrolytes (1:2) (1 molecule of metal with 2 molecules of ligand) derived to calculate the conductivity parameters, equivalent conductance at infinite dilution Λₒ, ionic conductivity, association constant Ka and the main distance between ions in solution( R) at best fit values of (6Λ). Thermodynamic quantities for the ion-association reaction ΔG°, Δङ° and ΔS°) have been also measured. The results of the analysis showed that the ions of the complex can be separated by solvent molecules (SSIP). The values of Ka, Λ° and R were found to be different from one solvent to another depending on the interactions in the solution. Furthermore, the chemical structure of the Proline-Mn complex was optimized by using of Gaussian interface version 16.0 program of chem3D to optimiz the features of complex molecule. The suggested statistical model possesses only two parameters with excellent values of the square regression coefficient (r2) and cross-validation (q2) are equal to 0.999 and 0.994, respectively, which refers to the perfect relationship between Ka value and the physical properties of the solvent.

A simple, fast, sensitive, and accurate spectrophotometric method has been developed for the determination of Chlorpheniramine maleate (CPM) in pure and pharmaceutical dosage forms, The quantitative determination of the drug applied used Zero-crossing order, third-order, and fourth-order derivative which measured at 262 nm,270 nm, and 264nm respectively. The methods obey Beers-Lambert law in the concentration range (1.95-39.07)µg/ml with a relative standard deviation RSD equal to 0.51% for the zero-order and 0.16% for the third-order and 1.94% for the fourth-order when the determination range was (1.95-7.81)µg/ml for the third-order with RSD equal to 1.37% for the integrated area under the positive peak, The developed spectrophotometric method in this study is successfully applied for the direct determination of the pharmaceutical form Histamine, The percentages recoveries were (96-98.6)% for the zero-order, (96.3-103.8) % for the third-order and (96.7-101.6) % for the fourth-order, this method is easy and economical and doesn't require the use of any expensive or toxic reagent; these advantages make it suitable for routine quality control.

The electrical conductivities of glutamic acid in the water, methanol, and ethanol are measured at 310.16 K and the conductivity parameters: Association constant (KA), equivalent conductance at infinite dilution (Λo) and the distance parameter (R). (Λo, KA, and R) are calculated. Values of Λo are found to be in the order: water > methanol > ethanol, but the order is reversed for the amount of KA and R. This indicates the increase of ion-solvent interaction and formation of solvent separated ion-pair in the above order. The main interest is to find an accurate yet efficient solvation model for semiempirical quantum-mechanical and Density Function Theory (DFT) methods applicable to amino acids (glutamic acid) in the context of computer-aided conductivity studying. It was reparametrization of the Conductor like Screening Model (COSMO) and Conductor-like Polarizable Continuum Model (CPCM) CPCM solvent model for Parameterization method three (PM3), Hartree-Fock (HF) & DFT calculation in Gaussian interface, version 16.0 which used to calculate many descriptors of the glutamic molecule and study the relationship between these descriptors and the association constant (KA). Molecular volume (MV), Connolly parameters, and the entropy were showed the same result corresponding with the experimental parameters.

The aim of this work is to measure the electrical conductivity of some complex of asparitic acid with (Fe, Co, Ni, Mn) at 310k in different methanol-water mixture, using Lee Wheaton equation of unsymmetrical electrolytes (1:2) to calculate the conductivity parameters (Λ º) equivalent conductance at infinite dilution, (º λ) ionic conductivity, (ΚΚ (association constant and (Ř) the main distance between ion in solution at best fit values and calculate the walden products (Λ º ŋ º) have also been calculated for each solvent composition to understand the solvation phenomena.

The present work involves a study of the polarographic behavior of pure Furosemide(lasimex) by direct method in aqueous solution , which gives a two well defined reduction peaks, the main peak at a potential (-0.196) volts and the other small peak at (-0.236) volts in phosphate buffer (pH=3).The calibration of Furosemide was constructed in phosphate buffer (pH=3) the calibration curves in the potential (-0.196) volts were observed in the range of concentration (6.83×10-5-9.90×10-5) M with a correlation coefficient (0.9884), and second in the range of concentration (10.51×10-5-12.34×10-5) M with a correlation coefficient (0.9919). The calibration curve of Furosemide in the presence of human blood serum was studied in the potential (-0.187) volts and range concentration of current (7.44×10-5-15.38×10-5) M with a correlation coefficient (0.9971). The calibration curve of Furosemide in presence of urine in the potential (-0.084) volt and range of concentration (7.42×10-5-12.31×10-5) M with a correlation coefficient (0.9960). The method was successfully applied to the determination of Furosemide in tablets in aqueous solution and in the presence of valium voltammetrically with out interferences.