Background: Diabetes mellitus type 2 (DMT2) is the most common type of diabetes and affects more than 90% of people with diabetes. Increasing age causes changes in carbohydrate metabolism and insulin release, which is influenced by blood sugar and inhibits the release of sugar into cells because it is influenced by insulin. The risk of diabetes mellitus increases with age, especially at 40 years and above. It happens because of the increase in sugar intolerance in people aged 40 and above.Objectives: This study aims to determine the description of serum creatinine levels in patients with type 2 diabetes mellitus at the age of 40 years and above.Methods: The research design is cross-sectional with inclusion criteria, namely DMT2 patients aged 40 years and above, and conducting examinations at the Prodia clinical laboratory, Banda Aceh branch, from January to May 2022. Samples were taken by accidental sampling, totaling 59 people. Data were collected by interview and laboratory examination to measure creatinine levels using the automatic analyzer method. The reference value for serum creatinine examination in men is 0,7-1,3 mg/dl and in women 0,6-1,1 mg/dl. Data analysis was done descriptively.Results: DMT2 patients aged 40 years and above were predominantly male (55,9%), and the frequency based on age range was mainly between 56-65 years (37,3%). As many as 16,9% of DMT2 patients aged 40 years and over had increased serum creatinine levels, although there were also 83,1% normal creatinine levels.Conclusion: DMT2 patients aged 40 years and above are dominant in men compared to women; the increase in creatinine levels do not show such a high value.

Background: Candida albicans is a fungus that requires organic compounds as a source of carbon and energy for growth and metabolic processes obtained from carbohydrates. One of the alternative media from materials that are readily available in nature but have not been widely utilized, namely from tubers such as white cassava (Manihot esculenta C.), which is believed to have a high carbohydrate content according to the needs of Candida albicans.Objectives: The study aims to determine whether white cassava can be used as an alternative medium for Candida albicans culture.Methods: The method used in this study is experimental, namely by seeing whether Candida albicans can grow or not on alternative media of white cassava. This study begins with the collection of Candida albicans specimens in patients with candidiasis, and then research is carried out at the Microbiology Laboratory of the Medical Laboratory Technology Department of the Aceh Ministry of Health Polytechnic. This research was carried out in 2020. The sample of this study was white cassava meat, as much as 150 grams. This study collected data by culturing Candida albicans on alternative media with raw materials for white cassava meat. For the identification of Candida albicans species, confirmation tests were carried out using the Germ tube test method. Furthermore, the data obtained from the assessment of colony growth (+) positive Candida albicans on white cassava media (Manihot esculenta C.) incubated for 72 hours (± three days) at 370C.Results: Based on the macroscopic and microscopic assessments, Candida albicans can grow well on white cassava media (Manihot esculenta C.).Conclusion: Alternative media of white cassava raw materials (Manihot esculenta C.) can be used as a culture medium for Candida albicans.

Background: Diabetes Mellitus is a group of metabolic disorders with typical symptoms of hyperglycemia. Blood glucose examination can use a spectrophotometer and a glucometer (Point of Care Test). Although both are used to check blood glucose, these two tools have some differences when viewed from the working principle, the samples used, and their benefits. Both tools have advantages and disadvantages. The cost factor, a spectrophotometer is more expensive and requires more blood and a long time. Compared to glucometers, which are cheaper, easier to use, and have faster results. However, the level of reliability in measuring the value of fasting blood sugar levels is not yet significantly known.Objectives: This study aims to measure the difference in fasting blood sugar levels based on a spectrophotometer examination with a glucometer examination at Sukajaya Health Center, Sabang City.Methods: The analytical descriptive research uses a cross-sectional design. The research was conducted at the Sukajaya Health Center in Sabang City in October 2019. The object of this study was 24 patients. The examination is carried out through a Duplo process to avoid errors. The tools used to check fasting blood sugar levels are Spectrophotometer and Glucometer. Data analysis used non-parametric statistical tests, namely Kruskal Wallis, at 95% CI.Results: The average fasting blood glucose level using a spectrophotometer with repetition twice (Duplo) is 146.83 mg/dl (minimum= 77 mg/dl and maximum= 404 mg/dl). While the average value of fasting blood glucose levels examined using a glucometer is 158,25 mg/dl (minimum = 66 mg/dl and maximum= 424 mg/dl). The results of statistical tests indicate a difference in the average fasting blood glucose levels in patients examined using a spectrophotometer with a glucometer (p= 0,019; p < 0,05) at UPTD Puskesmas Sukajaya Sabang City.Conclusion: Using a glucometer, the results of checking blood glucose levels show a higher value than a spectrophotometer. There are differences in fasting blood sugar levels in patients examined with a spectrophotometer and glucometer.

Bio-based industries require stable enzymes in a broad range of environmental conditions. Extremophiles have attracted more interests as the source of such enzymes, one of which is α-amylase. This study aimed to purify and characterize α-amylase produced by a thermo-halophilic bacterium PLS 75 isolated from underwater fumaroles. Ammonium sulfate precipitation results showed that the highest specific α-amylase activity (21.7 U/mg) obtained at 40-60% saturation level, with a purity of 7.7-fold of the crude extract with 16.2% yield. Further purification using DEAE Sepharose column chromatography increased the enzyme purity 11.1-fold of the crude extract with 7.1% yield. Specific activity after column chromatography purification was 31.3 U/mg. The pure enzyme had a low molecular weight of 14 kDa. The enzyme showed the highest activity at 80 °C and pH 5. The activity increased to 126% when in methanol, while decreased when in ethyl acetate and chloroform. The characteristics of α-amylase with low molecular weight, which was active in acidic condition, stable in polar and non-polar solvents, may be used for for specific industrial needs.