The present study examined the effects of foliar spray of selenium nanoparticles (0, 10 and 20 mg/L) on the yield, phytochemicals and essential oil content and composition of pineapple mint (Mentha suaveolens Ehrh.) under salinity stress (0, 30, 60 and 90 mM NaCl). Obtained results demonstrated that severe salinity stress reduced the fresh weight (FW) and plant height (PH) by 16.40% and 19.10%, respectively compared with normal growth condition. On the other hands, under sever salinity stress relative water content (RWC) and chlorophyll index were reduced by 18.05% and 3.50%, respectively. Interestingly, selenium nanoparticles (Se-NPs; 10 mg/L) application improved the pineapple mint growth. Based on GC-FID and GC-MS analysis, 19 compounds were identified in pineapple mint essential oil. Foliar application of Se-NPs and salinity did not change the essential oil content of pineapple mint, however, the essential oil compounds were significantly affected by salinity and Se-NPs- applications. Foliar application of Se-NPs- had a significant effect on piperitenone oxide, limonene, jasmone, viridiflorol and β-myrsene under different salinity levels. The highest percentage of piperitenone oxide (79.4%) as the major essential oil component was recorded in the no salinity treatment by applying 10 mg/L of nanoparticle. Interestingly, application of 10 mg L−1 Se-NPs- under 60 mM NaCl increased the piperitenone oxide content by 9.1% compared with non-sprayed plants. Finally, the obtained results demonstrated that foliar application of Se-NPs (10 mg L−1) can improve the pineapple mint growth and secondary metabolites profile under saline conditions.
Landraces of spinach in Iran have not been sufficiently characterised for their morpho-agronomic traits. Such characterisation would be helpful in the development of new genetically improved cultivars. In this study 54 spinach accessions collected from the major spinach growing areas of Iran were evaluated to determine their phenotypic diversity profile of spinach genotypes on the basis of 10 quantitative and 9 qualitative morpho-agronomic traits. High coefficients of variation were recorded in some quantitative traits (dry yield and leaf area) and all of the qualitative traits. Using principal component analysis, the first four principal components with eigen-values more than 1 contributed 87% of the variability among accessions for quantitative traits, whereas the first four principal components with eigen-values more than 0.8 contributed 79% of the variability among accessions for qualitative traits. The most important relations observed on the first two principal components were a strong positive association between leaf width and petiole length; between leaf length and leaf numbers in flowering; and among fresh yield, dry yield and petiole diameter; a near zero correlation between days to flowering with leaf width and petiole length. Prickly seeds, high percentage of female plants, smooth leaf texture, high numbers of leaves at flowering, greygreen leaves, erect petiole attitude and long petiole length are important characters for spinach breeding programmes.
Zinc (Zn) and copper (Cu) are essential micronutrients for the plant’s growth, development, and metabolism, but in high concentrations, the elements disrupt normal metabolic processes. The present study investigated the effects of different concentrations (added to a Hogland-based solution) of zinc (control, 5, 10 mg L−1 ZnSO4) and copper (control, 0.1, 0.2 mg L−1 CuSO4) on the growth characteristics and biochemical indices of summer squash (Cucurbita pepo L.). Compared with control, a single application of Cu or Zn at both concentrations significantly declined fruit yield, growth traits, pigments content, and high content of these minerals and values of stress-related indices. Increased Cu concentration in the nutritional solutions reduced the activity of ascorbate peroxidase (APX) and guaiacol peroxidase (GPX). Copper at high concentrations intensified ROS production, aggravated oxidative stresses, and decreased the plant yield and productivity. Nonetheless, combining Cu and Zn could alleviate stress intensity by boosting antioxidant enzymes, redox regulation, and a resultant diminishment in the content of H2O2, proline, malondialdehyde, and minerals. The obtained results corroborate that the co-application of zinc in Cu-contaminated areas can improve the plant’s economic yield and physiological parameters by hindering copper toxicity and enhancing the photosynthetic capacity.
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