Malasseziasp. causes skin diseases such as pityriasis versicolor, folliculitis, and atopic dermatitis. The present study aims to evaluate the antifungal activity ofJ. sambacor Arabian jasmine, a flowering plant abundant in the Southeast Asia againstMalasseziasp. using disc diffusion and broth microdilution method. The methanol extract and essential oil from the flowers and leaves ofJ. sambacwere, respectively, prepared using solvent extraction and hydrodistillation process. Skin samples from individuals with dandruff were cultured on Sabouraud dextrose agar overlaid with olive oil. The fungi that grew were observed microscopically, tested with Tween assimilation test, and cultured on CHROMagar (the chromogenic media pioneer) to identifyMalasseziasp. Out of 5 skin samples, only 2Malasseziasp. isolates were identified based on morphology and their ability to assimilate Tween. The inhibition zones of methanol extract of flowers and leaves ofJ. sambacand essential oil of flowers showed potential for antifungal activity with inhibition zones of 11.10 ± 1.92, 12.90 ± 1.68, and 13.06 ± 0.26 mm, respectively, and minimum inhibitory concentration (MIC) values of 80 mg/mL to 160 mg/mL and 50%, respectively. In conclusion,J. sambacmay be used as an alternative treatment againstMalassezia-associated skin infections.