No abstract

Saat ini di dunia sedang dilanda oleh sebuah penyakit yang disebabkan oleh virus SARS-CoV 2 atau yang dikenal dengan COVID-19. Pada orang lanjut usia dan memiliki riwayat penyakit kronis dapat mengakibatkan munculnya komplikasi yang lebih buruk. Seseorang dengan kadar glukosa darah tidak terkontrol, akan membuat prognosis COVID-19 semakin memburuk dan menyebabkan kematian. Puskesmas Trosobo, terdapat 50 anggota peserta prolanis yang aktif dengan kegiatan aktifnya setiap bulan. Saat pandemi Covid-19 kegiatan Prolanis terhambat. Kegiatan pengabdian masyarakat yang kami laksanakan bertujuan untuk mengetahui pentingnya peranan edukasi pola hidup sehat untuk mengkontrol kadar glukosa darah puasa anggota Prolanis di Puskesmas Trosobo selama pandemi Covid-19. Mayoritas anggota prolanis yang menjadi peserta kegiatan pengmas adalah berjenis kelamin perempuan, berusia 55-65 tahun, latar belakang pendidikan SMA, bekerja sebagai Ibu Rumah Tangga (IRT), dan tidak teratur dalam keteraturan kontrol berobat selama pandemi COVID-19. Pemberian edukasi pola hidup sehat pada masa pandemi COVID-19 terbukti efektif dan memberikan pengaruh yang signifikan mulai dari perubahan pola hidup sehat (p value = 0,043) dan penurunan kadar Glukosa Darah Puasa (p value = 0,043) pada anggota Prolanis Puskesmas Trosobo, Kabupaten Sidoarjo.

Farida Anwari 1), Melawati Olevianingrum 2), Umi Fatmawati 3) (Times New Roman, 11pt, bold)STIKES RS. Anwar Medika Sidoarjo, Jl. Bypass Krian KM 33 Balongbendo, Sidoarjofaridamph@gmail.com Hp. 08123248102 ABSTRACT           Polluted air and contaminated seeds can cause various lung diseases, one of which is indicated by cough changes. Coughing into body shape, occurs as a response from the body to irritation in the throat due to mucus, dust, smoke and air outside the body. Nebulizer is an alternative tool to relieve the cough response to the presence of mucus which is an inflammatory reaction due to infection in the respiratory tract. Mint oil in the form of extracts has a variety of esters, especially menthyl acetate and monoterpenes which produce a distinctive aroma that is beneficial for breathing. The aim of the study was to examine the effect of adding mint oil as a result of distillation on the liquid with the nebulizer in cough handling.       This study was a pre-experimental study with nonrandomized design pre test and post test with control group design which was used to determine the effect of the addition of mint oil as a result of distillation on the liquid with nebulizer to reduce the coughing status of the patient which included (cough frequency, onset of inflammation, wheezing, and ease of expectoration of phlegm).      The results showed that the cough frequency status is known to have decreased from 44.4% of patients to 23.5% of patients. Similarly, the status of inflammation decreased from 41.7% to 21.7%; wheezing status which was originally 58.3% decreased to 39.1%; easy  expectoration status also shows that mint oil can reduce the level of difficulty of phlegm to go out to 13% from 36.1%.

Abstract. Dengue is a disease caused by dengue virus infection. Diagnosis of the disease is often difficult to be upheld just by the symptoms. Therefore, laboratory diagnostics is required. The general objective of this study was to detect dengue IgG in serum samples with rapid tests and in urine samples of suspected dengue patients using ELISA. The procedure of this study consisted of three stages. First, collecting urine samples from healthy individuals and collecting blood and urine samples from suspected dengue patients; second, examining dengue IgG  in serum samples of suspected dengue patients with rapid tests; and third, examining dengue-specific IgG in urine samples from suspected dengue patients and from healthy individuals with ELISA technique. Data analyzed using experimental analytics to determine the cut off point used to interpret  laboratory diagnostic results. The results showed that patients with positive serum dengue IgG test at the same time also showed positive results on the examination of dengue-specific IgG in their urine samples while patients with negative serum at the same time showed negative results on the examination of dengue-specific IgG in urine samples.  Dengue-specific IgG examination in urine samples with ELISA technique can be used as an alternative examination in establishing the diagnosis of dengue, in which urine samples are taken from patients with more than 4 days fever.  Keywords: Dengue, IgG, Urine, ELISA   Abstrak. Dengue merupakan penyakit yang disebabkan virus dengue. Diagnosis penyakit ini sering sulit ditegakkan jika hanya melihat dari gejala. Oleh karena itu, diperlukan pemeriksaan laboratorium  untuk penegakkan diagnosis. Tujuan umum penelitian ini adalah untuk mendeteksi IgG dengue dalam sampel serum dengan rapid tes dan  pada sampel urine pasien terduga dengue dengan menggunakan metode ELISA. Prosedur penelitian ini terdiri dari tiga tahap. Pertama, pengumpulan sampel urine individu sehat dan pngumpulan sampel darah dan urine pada pasien terduga dengue; kedua, pemeriksaan IgG dengue dalam sampel darah pasien terduga infeksi dengue dengan rapid tes; dan ketiga, pemeriksaan IgG spesifik dengue pada sampel urine pasien terduga dengue dengan teknik ELISA, yang sebelumnya sudah dikelompokkan sesuai dengan hasil pemeriksaan  IgG dengue pada sampel serum dengan rapid tes, dan pemeriksaan IgG spesifik dengue sampel urine individu sehat dengan teknik ELISA.  Analitik eksperimental digunakan untuk menentukan cut off dari data yang kemudian digunakan untuk menafsirkan hasil diagnostik laboratorium. Hasil penelitian menunjukkan bahwa pada pasien dengan hasil pemeriksaan IgG dengue serum positif menunjukkan hasil positif juga pada pemeriksaan IgG spesifik dengue pada sampel urinnya, sedangkan pada hasil pemeriksaan serum yang negatif menunjukkan hasil negatif pula pada pemeriksaan IgG spesifik dengue di sampel urinnya. Pemeriksaan IgG spesifik dengue pada sampel urine dengan teknik ELISA dapat digunakan sebagai pemeriksaan alternatif dalam penegakan diagnosis dengue, di mana sampel urine diambil dari pasien dengan demam lebih dari 4 hari.  Kata Kunci: Dengue, IgG, Urine, ELISA

Severe dengue virus infection often causes proteinuria through several mechanisms, such as leakage of plasma proteins caused by higher protein filtering in the kidneys. The condition of proteinuria in patients with dengue shock syndrome will affect the management, prognosis, and mortality of patients with dengue virus infection. The objectives of this study were to determine the presence of protein in urine samples in patients in which IgG/IgM dengue was detected, to determine the presence of protein in urine samples in patients without dengue infection, and to determine the relationship between proteinuria in dengue patients and the degree of disease severity. The study was conducted in March-June 2020 at the Clinical Laboratory and Inpatient Clinic of Vita Medika Kepung, Kediri Regency. The method used was the consecutive sampling technique. The minimum sample size was 100 respondents with criteria for suspected dengue infection, onset fever > 4 days, age > 2 years, and no indication of other infectious diseases (blood and urine), divided into two groups, group with positive dengue infection and non-dengue group. The results showed that proteinuria was detected in 26% of respondents, secondary dengue infection and positive serological test of IgG, IgM only IgG were detected in 24 (92.3%) respondents and positive IgG and IgM were found in 2 (7.7%) respondents, this was shown by the p-value = 0.000 in the Chi-Square test with a Prevalence Ratio (PR) value of 11,987. In addition, Chi-Square test results indicated that a higher urine protein/albumin led to more severe disease, with p-value = 0.012 and 0.025 (p <0.05) and PR values of 3.333 and 2.800. The results of this study concluded that there was a close relationship between high levels of protein/albumin in the urine with the degree of disease severity in patients with dengue infection. In addition, an increase in urine protein/albumin levels is always followed by a decrease in serum protein/albumin levels.