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Ruminal cellulose digestion is a complex microbial process that involves adhesion of microbial cells to cellulose, cellulose hydrolysis, and fermentation of the resulting cellodextrins to volatile fatty acid, methane, and CO2 (Weimer, 1996). Information about how cellulose digestion and fermentation are affected by different ruminal environmental conditions is necessary to understand ruminant performance (Mourino, 2001). The aim of the present study was to elucidate the impact of type of supplemental non-structural carbohydrates (NSC) (starch and/or sucrose) on invitro first order disappearance kinetic of commercial cellulose (Cell).

Introduction Cellulose is the most abundant polymer in nature, but mammals do not synthesize enzymes to digest cellulose. Mosoni et al (2007) demonstrated that the dynamics of cellulolytic bacteria were in good correlation with the response to diet shift, particularly the changes of concentrate. The aim of the present study was to determine the effect of adding of non-structural carbohydrates (NSC) including sucrose, starch and equal mixture of them to an in vitro medium containing cellulose (Cell) as sole nutrient on both ruminal total anaerobic bacteria population and two major cellulolytic bacterial species (Fibrobacter succinogenes and Ruminococcus Albus) using SYBR Green real-time polymerase chain reaction (PCR) assay.