Background: Ocular hypertension is one of the most underdiagnosed ocular abnormalities among guinea pigs around the world.
Objectives:The current study investigates the effect of 0.0015% preservative-free tafluprost ophthalmic solution (Zioptan) on the intraocular pressure of 16 healthy male guinea pigs (Cavia porcellus) under different light/darkness regimes.Methods: All guinea pigs received a single drop of tafluprost at 5:30 in the right eye, whereas the contralateral eyes served as control to receive a placebo. Then, the animals were randomly divided into two groups; group A was exposed to light, whereas
Objectives
The purpose of this study was to evaluate conjunctival microflora and measure normal tear production and intraocular pressure (IOP) in two breeds of hedgehogs (long‐eared hedgehogs and Brandt's hedgehogs).
Methods
Forty‐eight hedgehogs from two different breeds were chosen for the study. Tear production was measured using the Schirmer tear test (STT) and phenol red thread test (PRTT) in both eyes. IOP was measured using a rebound tonometer. To perform microbiological sampling one drop of tetracaine was instilled in the eyes. Two sterile microswabs were used to collect samples for the microbial and fungal culture. All the microswab samples were transferred in phosphate‐buffered saline (PBS) to the laboratory for culture. Two MacConkey and two blood agar media plates were employed for each eye. Oneplate of sabouraud dextrose agar (SDA) was used for the fungal culture for each eye. Standard biochemical tests were performed to identify the isolated organisms.
Results
The mean STT and PRTT values were 1.6 ± 0.1 mm/min and 2.4 ± 0.3 mm/15 s in long‐eared hedgehogs and 2.2 ± 0.1 mm/min and 2.5 ± 0.3 mm/15 s in Brandt's hedgehogs, respectively. Mean (SD) Intraocular pressure of right eyes in long‐eared hedgehog and Brandt hedgehog were 19.7 ± 1.4 mmHg and 19.2 ± 2.4 mmHg, respectively. In the left eyes of long‐eared hedgehog and Brandt hedgehog mean (SD) IOP were 19.8 ± 1.5 mmHg and 19.5 ± 2.1 mmHg, respectively. In long‐eared hedgehogs, the most common bacteria were Staphylococcus epidermidis and Bacillus spp. In Brandt's hedgehogs, 24 out of 48 eyes had Staphylococcus epidermidis, which was the most commonly isolated bacterial species.
Conclusions
This study established reference intervals for IOP, STT and PRTT in hedgehogs and recognised and compared ocular conjunctival microflora in two breeds of hedgehogs.
OBJECTIVE
Periodontal disease is a common clinical complication and has a negative impact on the quality of life and the welfare of companion dogs. Periodontal disease occurs when pathogenic bacteria are accumulated in the gingival sulcus, which favors biofilm formation. The oral health of dogs can be significantly compromised by dental plaque accumulation. Thus, this investigation demonstrates the effect of Enterococcus faecium probiotic, dextranase enzyme, and their combination on dental biofilm in the oral cavity of dogs.
ANIMALS
The 30 dogs were referred to Polyclinic with no oral ulcers, severe periodontitis, and internal diseases.
PROCEDURES
Dextranase enzyme, E faecium probiotic, and their combination were administered in the oral cavity of dogs. Microbiological samples were obtained from tooth surfaces and gums before and after intervention with the substances. Bacterial colonies were enumerated by using a colony counter. Also, Porphyromonas gingivalis hmuY gene expression was evaluated by reverse transcription quantitative real-time PCR analysis.
RESULTS
The total colony count of the bacterial culture indicated that the dextranase enzyme, E faecium probiotic, and their combination significantly reduced the total bacteria count in the oral cavity. Moreover, in the reverse transcription quantitative real-time PCR analysis it was observed that using the combination of E faecium probiotic and dextranase enzyme decreases the hmuY gene expression of P gingivalis bacteria.
CLINICAL RELEVANCE
The results clearly indicated that the dextranase enzyme and E faecium probiotic could be used as preventive agents to reduce oral biofilm in dogs. Furthermore, no side effects were observed while using these substances.
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