The aim of the present research is evaluating the impact resistance of weft knitted fabrics which are knitted in basic patterns from the high tenacity Nylon 66. The woven fabrics have been applied for manufacturing technical and ballistic textiles so far. Although woven fabrics have been demonstrated satisfactory tensile properties, but they have not been resisted against impact, because of their poor strain against tensile forces. This research is important because knitted fabrics are applied in wide range of applications including technical textiles such as, package belts, safety belts, ballistic belts, and can be used to remove ice from airplane wings. Various knitted fabrics with different knitting elements such as knit, tuck and miss loops were produced. Mechanical properties including strength, work of the rupture and impact resistance of knitted samples were tested. The artificial neural network was used to predict mechanical properties of fabrics produced from the knitted structure as fitness function in genetic algorithm. After that, genetic algorithm was applied to find the optimum structure of knitted fabric with maximum impact resistance. The results of the genetic algorithm show that optimum structure of the fabric is cross-miss and rib structure with high stitch density.