Gender discrimination is a concept that is ever explained by law in detail in the workplace. It describes unequal advantages or disadvantages to a group in consideration of another group in the workplace. Gender discrimination affects the employees rapidly discrimination factor reducing increased satisfaction, motivational level, commitment to the organization, and stress level, increasing the working capability of employees in the organization. Gender discrimination factor which is in public and private organizations. 155 female employees of EEFU Humaya Takaful, University of Agriculture, Orient group of Company, Haier Group Company, and Ali Gohar are selected with the help of convenience sampling. Current research proposes the three hypotheses for direct relation and on for the mediational effect. All the hypotheses are accepted and the organizational environment plays a partial mediator between gender discrimination and job satisfaction.