Introduction: Epileptic neurological disorder, which is controlled with medications such as sodium valproate (one of the treatment priorities for the patients with epilepsy). Polycystic ovary syndrome (PCOS) is one of complication of sodium valproate. Ovulation in PCOs Patients is disrupted, resulting in serious complications, including endometrial hyperplasia with typical and atypical forms, increased the risk of endometrial endometrial cancer, diabetes mellitus, and decreased fertility rates. This study, in accordance with bioinformatics studies and other studies based on the association of miRNA-222 with genes involved in PCOs and hyperandrogenism, was designed to evaluate the association of PCOs in the patients treated with sodium valproate by simultaneously comparing the AMH factor and miRNA-222 marker at specific time periods. Methods: In this case-control study, 33 women with epilepsy before and after use the drug were selected based on inclusion and exclusion criteria. After blood sampling, their plasma was isolated. According to the instructions of the Total RNA extracted kit , cDNA synthesized and miRNA-222 expression was evaluated by RT-qPCR technique and statistical analysis was performed by SPSS 23 software, T-test and ANOVA tests. Results: The results of statistical tests such as T-test and ANOVA test (done with SPSS software) which showed a significant difference (p<0/01) between the mean expression of mir-222 and AMH in the patients before treatment compared to 3 month after treatment. The results of Pearson correlation test showed that the increase in AMH is directly related to the decrease in miRNA-222 expression (p<0/01). Conclusion: According to the results of the present study, in order to better control the possible side effects of the drug and possibly timely drug change and early diagnosis of PCOs (PCOs‌-like symptoms), evaluation of miRNA-222 expression changes can be used at the same time with AMH assay.

Introduction: Not being to get pregnant, after one year of unprotected sex is called infertility. In the post, infertility was mainly a female problem, but the role of male factors in infertility has denotation, although a greater percentage of this infertility is related to the deficiencies of semen. Methods: This case-control study was performed on sperm samples from 30 infertile oligoteratozoospermia (OT) patients as case group and 30 fertile and normosperm as controls, all the patients and controls between the ages of 20-49 years, who were referred to the Yazd Infertility Center for the treatment of infertility, were used to check the chromosomes 18, X and Y using the FISH technique. The results were considered using SPSS version 16 software and statistical tests T-TEST and Chi-Square Tests for statistical analysis and Pearson R correlation coefficient to measure the relationship between variables and statistical level P <0.05. Results: Disorders were observed in infertile oligoteratozoospermia men who were significantly related to the control group and between the chromosomal abnormalities, sperm counts and morphology )P<0/01) and there was also significant difference in correlation between chromosomal abnormalities and duration of infertility  (P<0/01). In addition, there was no correlation between chromosomal abnormalities and age, but the rate of chromosomal abnormalities in the age group of 40-49 years increased to 50%, which has the highest rate among age groups and definitely needs to be examined in a larger statistical population. Conclusion: This finding suggest that patients with OT may be at an increased risk of producing aneuploid offspring. Considering the chromosomal aneuploidy is recommended by cytogenetic molecular techniques.