Bisphenol A, an everywhere chemical, is applied as a plasticizer in polycarbonate plastics, which often used in our everyday products and in epoxy resins as protective coatings and linings for food and beverage cans for decades. Human exposure to BPA may lead to adverse effects by interfering with oestrogen receptors. Our present study was conducted to investigate the protective effects of selenium (Se) and vitamin E (Vit E) on BPA-induced damage in the liver of male rats. Animals were randomly divided into four groups: the first group received olive oil and served as control. The second group received both (Se + Vit E) (0.5 mg/kg diet; 100 mg/kg of diet). The third one treated orally by (10 mg/kg b.w.) of BPA. The last group received (Se + Vit E) (0.5 mg/kg diet; 100 mg/kg of diet) concomitantly with (10 mg/kg b.w.) BPA. Exposure to BPA for three weeks engendered a hepatic disorder. An increased AST and ALT enzymatic activity was noticed in BPA-treated group as compared to other groups. Furthermore, a change in glucose, cholesterol, LDL-C, HDL-C, albumin, and bilirubin level was remarkable. Moreover, exposure to BPA increased malondialdehyde levels while reduced gluthatione content was decreased in the liver homogenate. A decrease in glutathione peroxidase, glutathione s-transferase and catalase activities was observed in the same group. Administration of selenium and vitamin E through the diet in BPA treated rats ameliorated the biochemical parameters cited above. In addition, an improvement in activities of liver enzymes was recorded. The histological findings confirmed the biochemical results. The model of this study that we employed characterized the relationships between BPA-induced hepatotoxicity and its alleviation by natural antioxidants like selenium and vitamin E.

Introduction: The aim of this study is to throw light on the possible protective effect of pomegranate juice (GJ) (Punica granatum L) after experimental oral administration of fungicide Thiophanate Methyl (TM) and its effects on sperm quality, of male healthy Wistar rats. Materials and Methods:  Fifteen healthy adult male Wistar rats were divided into 03 groups. Group 1: control, Group 2: treated with Thiophanate Methyl (TM) (150 mg / kg) and Group 3: Treated with Thiophanate Methyl + pomegranate juice (GJ) (150 mg/kg + 1.5 ml). The method used for the treatment is oral gavage daily, for 60 days. The reproductive organs that were collected were weighed, and the epididymis and testis were used for further evaluations (count, viability, motility of spermatozoa), we also conducted histological tests for testis and epididymis. Results: The results showed that the treatment with Thiophante Methyl induced a decrease in concentration, motility, and viability of spermatozoa while the treatment of rats with fungicide associated with the grenade juice restored the concentration and the mobility of spermatozoa. Conclusion: Thiophanate Methyl (TM) caused structural changes in the epididymis and testes inducing a decrease in sperm quality, which would interfere with its function and contribute to infertility. On the other hand, GJ can prevent these changes by increasing the number of caudal sperm; thus, it could have an effect in improving male fertility.