Background: Wound infection is one of the public health problems worldwide associated with the colonization of bacterial pathogens and resistance to commonly used antibiotics. The study aimed to identify the most common bacterial pathogens that cause wound infections and their antimicrobial susceptibility of isolates from patients at shaafi Hospital in Mogadishu, Somalia. Methods: This study was a descriptive cross-sectional design conducted from January to June - 2020 at shaafi Hospital in Mogadishu –Somalia. A total of 80 patients were taken from wound swab; all specimens were directly cultured on standard media, then Gram stain and Biochemical tests were also done antimicrobial susceptibility test was performed following Standards guidelines and procedures of (CLSI). Results: A total of 80 patients were taken from the wound swab; of these, 25 samples shown significant growth, isolating 38 bacteria strains. The most bacteria that isolated was Staphylococcus aureus 15 (39.47%), followed by Escherichia coli 12 (32%), Klebsiella pneumonia 8 (21%), Proteus Vulgaris 2 (5%), and Pseudomonas aeruginosa 1(3%). Staphylococcus aureus was entirely resistant to penicillin G, Ampicillin, and Methicillin, less sensitive to amoxicillin, and strongly sensitive to gentamicin. Moreover, All isolates of Escherichia coli, Klebsiella pneumonia, Proteus Vulgaris were sensitive to gentamicin and norfloxacin. In contrast, all isolates of Pseudomonas aeruginosa were resistant to gentamicin and norfloxacin. Conclusions: The study revealed that bacterial pathogens of wound infection were resistant to commonly used antibiotics in Mogadishu, Somalia, showing an increasing trend of antibiotic-resistant. The study suggested that timely diagnosis with antimicrobial susceptibility is necessary before prescribing appropriate antibiotics.