This study aimed to establishment database and baseline of hemato-biochemicals values of male and female camels in three governorate south Iraq (Basrah, Dhi qar, and Muthana). Forty eight adult male and female dromedary camels (6 males and 10 females for each governorate) aged over 3 years old were used. Peripheral blood samples were collected from the jugular vein during February – April 2018 and then as soon as transported to the laboratory for haematology ( RBC, Hgb, HCT, MCV, MCH, MCHC, ESR, PLT,and total and differential WBC) and biochemical analysis(ALT, AST, Glucose, Creatinine, Urea, and total protein). The results of haematological and biochemical parameters appeared compatible with the previous studies on normal hemato-biochemical values and found significant effect of sex on some haematological parameters of camel especially for RBCs count, Hgb, PCV, MCV, and PLT, and also among the camels of different governorate in south of Iraq for both sexes regarding to haematological parameters especially in Basrah governorate compared with Dhi qar and Muthana. Whereas, no effect were recorded for sex of camels on total and differential leukocytes in all location studied. Rather than significant differences (p<0.05) showed among the governorates in values of total and differential leukocytes for both sexes compared with their counterparts in the other governorates. The serum biochemical study of camels’ revealed no effect for sex on most of biochemical parameters except in some situation for AST activity level and glucose concentration. In contrast, the results revealed significant variation for both sexes compared with their counterparts in other studied governorates especially in AST, ALT, and glucose values. Therefore, the present study concluded that the results compatible with the previous studies and the sex had effect on some blood indices and the hemato-biochemical parameters different in their value depend on location in south region of Iraq.