Three-dimensional (3D) bioprinting systems, which are the prominent tools forbiofabrication, should evolve around the cutting-edge technologies of tissue engineering.This is the case with organoid technology, which requires a plethora ofnew materials to evolve, including extracellular matrices with specific mechanicaland biochemical properties. For a bioprinting system to facilitate organoid growth, itmust be able to recreate an organ-like environment within the 3D construct. In thisstudy, a well-established, self-assembling peptide system was employed to generatea laminin-like bioink to provide signals of cell adhesion and lumen formation incancer stem cells. One bioink formulation led to the formation of lumen with outperformingcharacteristics, which showed good stability of the printed construct.