Background: Adolescence is a critical life phase in which individuals must have the opportunity to develop the capabilities required for realizing their full potential and achieving a healthy and fulfilling life. Healthy adolescence is the foundation of a healthy adult and this therefore becomes an important phase to concentrate upon by all the concerned stakeholders. The aim of the study: to assess the mothers' perception concerning their adolescent daughters' seeking for gynecological health services. Design: A descriptive study design was used. Setting: The study was conducted at outpatient clinics in EL Menofieya University Hospitals. Sample: A purposive sample of (385) mothers of adolescent girls were recruited on the study. Data collection tools: Structured interviewing questionnaire sheet and a three points Likert scale. Results: The result of the present study reveals that 68.8% of the studied mothers has unsatisfactory knowledge about their adolescent daughters' seeking for gynecological health services. The more than two third of the sample had positive attitude concerning their adolescent daughters' seeking for gynecological health services. Conclusion: It is concluded that more than two third of the studied mothers have unsatisfactory knowledge about adolescence gynecological problems, causes of gynecological problems, adolescent's complications relating to ignoring any gynecological problem, organs that examined during gynecological examination for adolescent, and centers for treating gynecological problems respectively. Recommendation: Conduction of awareness secessions for mothers' of adolescent girls to improve their knowledge about adolescent daughters' gynecological health problems and services Future research should focus on effect of instructional guideline about adolescent daughters' gynecological health problems and services on their mothers' knowledge and attitude.