Arduino is a tool which the data taken from sensors can be used. Temperature, humidity, distance, light, color and acceleration can be measured by Arduino connected sensors. It is aimed to determine the effects of analytical chemistry experiments supported by Arduino on pre-service teachers' self-efficacy toward information technologies. Participants of this study are 61 pre-service teachers. There are one experimental group (n EG :34) and the other control group (n CG :31) with random assignment method. In this study, "pre-test-post-test control group semiexperimental research design" has been adopted. In the experimental group, closed-ended experiments supported by Arduino were used. In the control group, closed-end experiments not supported Arduino were used. In both groups, confirmatory approach based experiment design was adopted. In order to measure self-efficacy perceptions of information technologies, "Information Technologies Self-efficacy Perception Scale for Pre-service Teachers (ITSPS)" was used. According to the results of the study, it was determined that the analytical chemistry experiments supported by Arduino has effects on their technology self-efficacy in a positive way. Arduino should be assessed on students' self-efficacy by testing in different chemistry-related trials. In addition, easier application of Arduino should be investigated.