Purpose: This study was designed to evaluate the impact of irrigating solutions on push out bond strength of Bio-ceramic and resin based endodontic sealers. Materials and Methods: sixty extracted human teeth with single root and single canal were prepared with RACE FKG rotary files (#35, 0.06) taper. The specimens were divided into two experimental groups according to the final rinse used (30 samples each): Group (I) irrigated with 17%EDTA, Group (II) irrigated with 2% Chlorohexidinedigluconate (CHX). Each group was subdivided into two subgroups according to the sealer used (15 samples each). Total Fill Bio-ceramic sealer, and AH Plus Sealer The specimens were obturated by single cone gutta-percha with vertical condensation technique. Results: Final irrigation with EDTA resulted in the highest bond strength of bioceramic sealer to dentin of the root which was statistically different from irrigation with chlorohexidinedigluconate (CHX). Bond strength values were as follows: EDTA>CHX. Conclusion: The type of final irrigation affects the adhesion of Total Fill Bio-ceramic and AH plus sealers to root canal dentin.