-It was studied the effects of shading (0, 36 and 54%) and of four nitrogen doses (0, 50, 100 and 150 mg N/dm 3 of soil) on the morphogenetic traits and on the tillering of grasses of Brachiaria genus (B. decumbens, B. ruziziensis and B. brizantha cv. Marandu and cv. Xaraes), cultivated in pots with 5 kg substrate. It was used a complete random design, in a 3 × 4 × 4 factorial scheme with three replications. Nitrogen promoted increase in leaf appearance rate and in average leaf blade length, whereas shading increased stem elongation rate. Leaf elongation rate and tillering varied with the interaction among shading percentages and nitrogen doses. Shading increased response efficiency of leaf elongation rate to nitrogen doses, but reduced for tillering, evidencing priority existing tillering growth in detriment to appearance of new tillers when there is light restriction for the plants. Phenotypic adjustments, in response to light reduction, indicate that the studied grasses present tolerance to moderate shading.
Pasture is the main food source for more than 200 million cattle heads in Brazil. Although Brazilian forage breeding programs have successfully released well-adapted, high-yielding cultivars over the years, the use of genomic tools in these programs is currently limited. These tools are required to tackle the main challenges for tropical forage breeding in Brazil. In this context, this note lists the main research priorities raised at the workshop "Breeding Forages in the Genomic Era", which are necessary to accelerate the use of genomic tools for next-generation breeding of tropical forages and allow breeders to increase genetic gains. Additionally, an online discussion forum (hosted at http://www. cnpgl.embrapa.br/genfor) has been launched to strengthen collaborations among research groups. The research priorities and more synergistic collaborations will assist researchers and decision-makers in delivering a sustainable increase in production of animal products, especially beef and milk, which are required to feed a rising world population.
Resumo -O objetivo deste trabalho foi avaliar genótipos de capim-elefante (Pennisetum purpureum Schum.) quanto à resistência à cigarrinha-das-pastagens (Mahanarva spectabilis). Para avaliação da antibiose, aos trinta dias após o plantio, cada planta foi infestada com seis ovos próximos à eclosão, em delineamento inteiramente casualizado, com 30 genótipos e dez repetições. Quarenta e cinco dias após a eclosão das ninfas, avaliou-se a porcentagem de sobrevivência do inseto-praga nos diferentes genótipos. Para avaliação da não-preferência, foram quantificados, quinzenalmente, o número e tamanho de ninfas por vaso, em plantas mantidas em casa de vegetação, onde adultos de M. spectabilis eram periodicamente liberados, em delineamento inteiramente casualizado, com três repetições por genótipo, em dois períodos de amostragem. Os genótipos Cameroon de Piracicaba, Pioneiro, Cuba 169, Santa Rita, Mineiro Ipeaco, Mercker Comum de Pinda e CNPGL 96-27-3 foram selecionados quanto à resistência, pelo mecanismo de antibiose. O número e o tamanho médio das ninfas variaram significativamente em razão do genótipo de capim-elefante, no estudo da não-preferência. Os genótipos Roxo de Botucatu e Pioneiro são candidatos à testemunha suscetível e resistente, respectivamente, pelo mecanismo de antibiose, e os genótipos Cameroon e Cameroon Piracicaba são promissores pelo mecanismo de não-preferência.Termos para indexação: Pennisetum purpureum, Mahanarva spectabilis, resistência a pragas, forrageiras, antibiose, não-preferência. Selection of elephant grass genotypes for resistance to spittlebugAbstract -The objective of this work was to evaluate genotypes of elephant grass (Pennisetum purpureum Schum.) regarding resistance to the spittlebug Mahanarva spectabilis. For antibiosis evaluation, each plant was infested with six eggs near hatching, thirty days after planting, in a completely randomized design with 30 genotypes and ten repetitions. Forty-five days after the nymphs hatched, the insect survival rate was evaluated on the different genotypes. For nonpreference mechanism evaluation, the size and number of the nymphs per pot were assessed every 15 days, in plants kept at greenhouse, where adults of M. spectabilis were periodically released, in a completely randomized design, with three repetitions per genotype in two sampling periods. Cameroon de Piracicaba, Pioneiro, Cuba 169, Santa Rita, Mineiro Ipeaco, Mercker Comum de Pinda and CNPGL 96-27-3 genotypes were selected for resistance, by antibiosis mechanism. In the study of the nonpreference mechanism, the number and average size of the nymphs varied significantly as a function of the elephant grass genotype. Roxo de Botucatu and Pioneiro genotypes are, respectively, susceptible and resistant check candidates by the antibiosis mechanism, and Cameroon and Cameroon Piracicaba genotypes are promising by the nonpreference mechanism.
-The objective of the present work was to induce chromosome doubling in genotypes of Brachiaria ruziziensis with potential for production of good-quality biomass and tolerance/resistance to stress caused by aluminum and spittlebugs. Germinated seeds of B. ruziziensis were immersed in 0.1% colchicine solution for 2 or 3 hours. Ploidy level was determined by flow cytometry and confirmed by chromosome count. Fertility of the tetraploid plants was assessed through evaluation of pollen viability by staining. There was no statistical difference with regard to seedling survival and number of duplicate seedlings between the treatments. Survival rate was 8%, of which 11.45% were tetraploid genotypes. The mean viability of pollen grains from the evaluated tetraploid plants ranged from 51.75 to 55.50%. So, the tetraploids plants obtained from genotypes of a bred population of ruzigrass produce fertile pollen, in percentages that render crosses viable in genetic breeding programs.
RESUMOObjetiva-se com este trabalho estimar a repetibilidade para caracteres forrageiros de Panicum, e determinar o número de cortes de avaliação necessários para a seleção de genótipos de Panicum, com confiabilidade. Utilizaram-se os dados de um ensaio conduzido no período de 21/11/2002 a 08/04/2005, no Campo Experimental da Embrapa Gado de Leite, localizado em Valença-RJ, onde foram realizados 15 cortes de avaliação. No ensaio, foram avaliados 23 genótipos de Panicum maximum, em parcelas experimentais, dispostas no delineamento de blocos casualizados, com três repetições. Foram estimados os coeficientes de repetibilidade para as características produção de matéria verde de forragem (PMV); produção de matéria seca de forragem (PMS) e de folhas (PMSF); porcentagem de folhas na PMS (%FOL) e altura da planta (AP), utilizando os métodos da análise de variância, componentes principais e análise estrutural. Para todas as características avaliadas os efeitos de genótipos, cortes e interação genótipos x cortes foram significativos (P<0,01). Quando se considerou o coeficiente de determinação de 85%, o número de avaliações (cortes) necessários para a determinação do valor real dos genótipos foram de 10, 9, 7, 11 e 3, respectivamente, para PMV, PMS, PMSF, %FOL e AP. O método dos componentes principais e o da análiseestrutural (baseado na matriz de correlações) foram concordantes para todas as características avaliadas. A realização de 10 cortes de avaliação permite discriminar o valor real dos genótipos de Panicum, com confiabilidade superior a 85%, para a maioria das características avaliadas.Termos para indexação: Forrageira, gramínea, componentes principais, análise estrutural, coeficiente de repetibilidade. ABSTRACTThe objective of this work was to estimate the repeatability for forage characters of Panicum and to determinate the necessary number of evaluation cuts to select Panicum genotypes with confidence. Data of a trial with 15 cuts, carried out between 21/11/2002 and 08/04/2005 in the experimental station of Embrapa Gado de Leite located in Valença, RJ, Brazil, were used. In this study. 23 genotypes of Panicum maximum were evaluated, in a complete randomized block, with three replications. The coefficient of repeatability for fresh forage production (PMV), total plant dry matter production (PMS) and leaves dry matter production (PMSF) were recorded along with leaves percentage in PMS (%FOL) and plant hight (AP), using the variance analysis, main components and structural analysis methods. For all evaluated parameters the effects of genotype, cut and genotype x cut interaction were significant (P<0.01). When considering the determination coefficient as 85%, the required number of measures (cuts) to determine the real value of genotypes were 10, 9, 7, 11 and 3, for PMV, PMS, PMSF, %FOL and AP, respectively. Main components and structural analysis methods (based on the correlation matrix) did agree for all features evaluated. The utilization of data from 10 cuts allows to discriminate, with confidence higher than 8...
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