FIGURE 1. Maximum likelihood phylogram including Collybiopsis pakistanica (GenBank accession no: OP199106, OP199107), inferred from ITS dataset with two taxa of Omphalotus used as outgroup. Maximum likelihood bootstrap values are given on the nodes. The newly obtained sequences are highlighted in bold.

Marasmius margallensis sp. nov. of sect. Sicci from Margalla hills, Islamabad, Pakistan, is described and illustrated, based on morphological characteristics and multigene phylogenetic analyses. The taxon is characterized by a pale yellow to dull orange pileus with light gray margins, clavate to narrowly fusiform basidiospores, rather short pleurocystidia and hymeniderm pileipellis with Siccus-type broom-cells. Marasmius pulcherripes is closest relative of the newly described species M. margallensis in sect. Sicci in phylogenetic analyses but the later one is distinct by macro-, and micro-morphological features and molecular data.

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Two species of the genus Laccaria, are described as new reports for Pakistan. Laccaria murina has been collected from a Himalayan moist temperate forest in Khanspur, KP, while L. pumila was found in the moist temperate forests of Kumrat Valley in Dir Upper, KP, and at higher altitudes of the Deosai plains of Gilgit-Baltistan, Pakistan.Phylogenetic analysis based on the nrITS region clustered the Pakistani species with L. murina sequences with other Asian collections and L. pumila from USA and Netherlands. SEM of basidiospores along with detailed micro-morphological data are provided. General distribution, habitat, ecology, and diagnostic features are also discussed. Research Highlights• It has new reports from Pakistan, studies based on light, scanning electron microscopy, and nrITS molecular markers.• These species have been described with detailed micro-morphological and molecular phylogenetic analyses.• General distribution, ecology, diagnostic features, and comparisons with closely related specimens have been provided.• Graphical representation of DNA extraction and geographical locations of sampling sites are also illustrated (Figures 1 and 2).• Very few members of this genus are already described from Pakistan.

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